FastQCFastQC Report
Thu 26 May 2016
ERR580248_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580248_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9285913
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT266200.28667078832205295No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207020.22293984447194368No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130280.14029853607286652No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA141150.046.052121
GTATCAA252050.040.904991
CAACGCA281100.035.150125
ATCAACG288700.034.2462543
TATCAAC313600.032.6971552
GTACTAG75900.032.2863121
AACGCAG325200.031.1768196
GTGGTAT45150.029.898311
TCAACGC336750.029.7774854
GCATCGT77500.028.6247778
GTACATG353450.028.4513031
ACGCAGA347950.028.3673217
CGCAGAG358350.027.5038078
TAGGCAT93800.027.2244195
TACATGG363500.027.1346042
ACATGGG354350.026.8263173
GGGCACC85200.026.75364194
CTAGGCA102900.025.9621944
TAGTACT102650.025.9337844
CTGTGCG102750.025.2966969
Produced by FastQC (version 0.11.5)