FastQCFastQC Report
Thu 26 May 2016
ERR580248_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580248_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9285913
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225660.24301326105467497No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181460.19541427967287653No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124110.13365406287997744No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA139000.045.7238581
GTATCAA243050.039.7076641
CAACGCA278900.033.0461775
ATCAACG288250.032.056123
GTACTAG73450.031.1046621
TATCAAC313900.030.6656232
AACGCAG319900.029.4123886
TCAACGC333700.028.1265434
TAGGCAT94750.027.381175
GTACATG355450.027.0323241
GGGCACC87550.027.0068694
ACGCAGA346000.026.623217
GTGGTAT45100.026.5793691
TACATGG356200.026.5225522
TAGTACT99300.026.5053314
CTGTGCG101250.026.0868559
CGCAGAG356350.025.8895198
CTAGGCA105600.025.6361644
ACATGGG352600.025.592683
CTAACGC31800.025.4213223
Produced by FastQC (version 0.11.5)