FastQCFastQC Report
Thu 26 May 2016
ERR580247_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580247_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4703127
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237090.5041114135340168No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192480.4092596266271355No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114050.24249823574825857No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71020.15100591585130488No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98450.054.5426451
GTATCAA183850.041.611151
TACCTGG30200.035.8101962
TAACGCA6800.035.262634
ATCAACG221850.033.9304773
CAACGCA224100.033.6082845
TCAACGC225650.033.4003944
TATCAAC228650.032.9851842
TATAACG7900.032.735682
AACGCAG235500.032.38276
ATAACGC7950.030.753483
ACGCAGA261850.028.9792377
CGCAGAG265500.028.421878
TATCACG4650.028.3133242
GTACATG170150.027.2477551
GTACCTG41950.026.6766171
GCAGAGT285350.025.8739249
TACATGG178250.025.3237192
ACCTGGG41250.025.3038773
ACATGGG172250.025.248793
Produced by FastQC (version 0.11.5)