FastQCFastQC Report
Thu 26 May 2016
ERR580247_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580247_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4703127
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210960.44855263317363103No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181380.38565830775992227No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108530.23076136366294173No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63520.13505907877886353No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97150.054.6533281
GTATCAA177400.040.0040441
TACCTGG31200.035.5485152
TATCACG4450.033.7951242
ATCAACG217800.031.8710163
CAACGCA219550.031.5952365
TCAACGC222300.031.2681394
TATCAAC224300.031.0724812
AACGCAG232000.030.2627376
TAACGCA7700.028.686694
ACCTGGG38350.028.6763443
ATAACGC7900.027.3655413
TATAACG8700.027.0093922
GTACATG171800.026.8982451
ACGCAGA259500.026.7662357
CGCAGAG264700.026.133898
GTACCTG45000.025.6728781
TACATGG178500.025.170032
ACATGGG175450.024.7777393
CATGGGG111650.023.866994
Produced by FastQC (version 0.11.5)