FastQCFastQC Report
Thu 26 May 2016
ERR580246_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580246_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9580347
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300860.3140387294948711No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250440.26141015560292336No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144350.15067303929596704No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA147600.051.7087551
GTATCAA266900.041.4577061
CAACGCA318850.033.901485
ATCAACG318400.033.8783873
TATCAAC336900.032.5785522
TCAACGC335300.032.3526344
AACGCAG344000.031.9391296
ACGCAGA383000.028.1824557
CGCAGAG396200.027.1603498
GTGGTAT64350.026.307881
GTACATG307950.025.6238061
TACATGG314850.024.405082
GCAGAGT434500.024.2072739
ACATGGG314250.023.4774043
TGGTATC70750.023.2634162
CATGGGG231600.021.9067764
GTACTAG39750.021.6493991
GAGTACT269900.021.36268812-13
CAGAGTA426550.020.49575610-11
AGAGTAC398250.020.45921710-11
Produced by FastQC (version 0.11.5)