FastQCFastQC Report
Thu 26 May 2016
ERR580246_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580246_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9580347
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271970.28388324556511363No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240580.25111825281485106No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139200.14529745112572645No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA148650.054.1177861
GTATCAA270850.041.4008181
CAACGCA326850.033.2016375
ATCAACG327250.033.0897673
TATCAAC345650.032.129032
TCAACGC346500.031.4272844
AACGCAG354600.031.0258796
GTGGTAT64600.027.3001461
ACGCAGA399250.027.2148887
CGCAGAG409250.026.4809918
GTACATG309650.024.6091271
GTACTAG40050.024.3721431
TACATGG314600.023.6030882
GCAGAGT455950.023.3873449
ACATGGG315350.022.4451753
TGGTATC72000.022.3235912
GAGTACT281250.021.43788312-13
CATGGGG233150.021.14584
CAGAGTA439450.020.580310-11
AGAGTAC413700.020.50950810-11
Produced by FastQC (version 0.11.5)