##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR580246_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9580347
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.53101698717176	34.0	31.0	34.0	31.0	34.0
2	32.732012107703405	34.0	31.0	34.0	31.0	34.0
3	32.809935276874626	34.0	31.0	34.0	31.0	34.0
4	36.280568960602366	37.0	37.0	37.0	35.0	37.0
5	36.21499367402872	37.0	37.0	37.0	35.0	37.0
6	36.24418520540018	37.0	37.0	37.0	35.0	37.0
7	36.21057243542431	37.0	37.0	37.0	35.0	37.0
8	36.201609816429404	37.0	37.0	37.0	35.0	37.0
9	37.996834665800726	39.0	38.0	39.0	35.0	39.0
10-11	37.94962922532973	39.0	38.0	39.0	35.0	39.0
12-13	37.9133818430585	39.0	38.0	39.0	35.0	39.0
14-15	39.31756981245043	41.0	39.0	41.0	36.0	41.0
16-17	39.30467878668695	41.0	39.0	41.0	36.0	41.0
18-19	39.31424086204811	41.0	39.0	41.0	36.0	41.0
20-21	39.30283850887656	41.0	39.0	41.0	36.0	41.0
22-23	39.229192324662144	41.0	39.0	41.0	36.0	41.0
24-25	39.15105329692129	41.0	39.0	41.0	35.5	41.0
26-27	39.0146825579491	40.0	38.5	41.0	35.0	41.0
28-29	38.901720835372664	40.0	38.0	41.0	35.0	41.0
30-31	38.77580170112836	40.0	38.0	41.0	35.0	41.0
32-33	38.61571219706343	40.0	38.0	41.0	35.0	41.0
34-35	38.56940651523374	40.0	38.0	41.0	34.5	41.0
36-37	38.59745273318388	40.0	38.0	41.0	35.0	41.0
38-39	38.49744242040502	40.0	38.0	41.0	34.5	41.0
40-41	38.366312097046176	40.0	38.0	41.0	34.0	41.0
42-43	38.326548245068786	40.0	38.0	41.0	34.0	41.0
44-45	38.225027966106026	40.0	38.0	41.0	34.0	41.0
46-47	38.00718053323121	40.0	38.0	41.0	33.0	41.0
48-49	37.78037209925695	40.0	37.0	41.0	33.0	41.0
50-51	37.605049065550546	40.0	37.0	41.0	33.0	41.0
52-53	37.43393746593939	40.0	36.5	41.0	32.5	41.0
54-55	37.241734980998075	40.0	36.0	41.0	32.0	41.0
56-57	37.03759253187802	39.0	35.5	41.0	31.5	41.0
58-59	36.81586261958988	39.0	35.0	41.0	31.0	41.0
60-61	36.5664497329794	39.0	35.0	41.0	31.0	41.0
62-63	36.28778607914724	38.0	35.0	40.0	31.0	41.0
64-65	36.0007338460705	37.5	35.0	40.0	30.5	41.0
66-67	35.67610426845708	37.0	35.0	40.0	30.0	41.0
68-69	35.323943015842744	36.5	35.0	39.0	29.5	41.0
70-71	34.98996205461034	36.0	34.0	39.0	29.0	41.0
72-73	34.63247839561552	35.5	34.0	38.5	29.0	40.0
74-75	34.27223679893849	35.0	34.0	37.5	29.0	40.0
76-77	33.032673346800486	34.5	32.5	36.0	27.0	39.0
78-79	33.650460729658334	35.0	33.5	36.5	29.0	39.0
80-81	33.573564715349036	35.0	34.0	36.0	29.0	38.5
82-83	33.348965804683274	35.0	34.0	36.0	29.0	37.0
84-85	33.11595968287996	35.0	34.0	35.5	29.0	37.0
86-87	32.926125588144146	35.0	33.0	35.0	28.0	36.5
88-89	32.74710159245798	35.0	33.0	35.0	27.0	36.0
90-91	32.57494759845338	35.0	33.0	35.0	27.0	36.0
92-93	32.449356375087454	35.0	33.0	35.0	27.0	36.0
94-95	32.33921673192005	35.0	33.0	35.0	27.0	35.0
96-97	32.235059126772754	35.0	33.0	35.0	26.0	35.0
98-99	32.15126670255263	35.0	33.0	35.0	25.5	35.0
100	30.89672169494487	34.0	30.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.12409463447599478
1101	2	0.06593419728664429
1101	3	-0.0014581141176535084
1101	4	0.03306531804533819
1101	5	-0.010165149373854376
1101	6	0.0015119001663208564
1101	7	-0.026854776943324055
1101	8	0.006608365636736835
1101	9	-0.01730127111373747
1101	10-11	0.004092499653019388
1101	12-13	-0.048823526827611374
1101	14-15	-0.015469083630783587
1101	16-17	-0.07780763471587449
1101	18-19	-0.05225217225895307
1101	20-21	-0.08777157550278503
1101	22-23	-0.09975262026829768
1101	24-25	-0.0986687149389951
1101	26-27	-0.08388231471510466
1101	28-29	-0.08258593453703611
1101	30-31	-0.11157032865905592
1101	32-33	-0.09841979754570218
1101	34-35	-0.1322902423005985
1101	36-37	-0.08036029551126944
1101	38-39	0.01997801965428181
1101	40-41	0.030286907557751874
1101	42-43	-0.022946155385035638
1101	44-45	-0.044273000980965094
1101	46-47	0.04742229123681341
1101	48-49	-0.04748444410331132
1101	50-51	-0.040772387484757644
1101	52-53	-0.06852519441987681
1101	54-55	-0.07144438994052393
1101	56-57	-0.03718389144678724
1101	58-59	-0.09313759220946594
1101	60-61	-0.1711144476419264
1101	62-63	-0.11754358044642288
1101	64-65	-0.03255662617272037
1101	66-67	-0.08090048189504984
1101	68-69	-0.09029321930997725
1101	70-71	-0.1039223276881529
1101	72-73	-0.09475534724727197
1101	74-75	-0.1037941238159803
1101	76-77	-0.13311498733898475
1101	78-79	-0.10490317821217587
1101	80-81	-0.0915376187525041
1101	82-83	-0.04837574028164937
1101	84-85	-0.02401360941222208
1101	86-87	-0.05523211035370679
1101	88-89	-0.021192116685703866
1101	90-91	-0.05035848811380106
1101	92-93	-0.15114034888394912
1101	94-95	-0.1795483615922464
1101	96-97	-0.15310649542273325
1101	98-99	-0.17269846224974117
1101	100	-0.0752793311366382
1102	1	0.11044703910632592
1102	2	0.025855340074627975
1102	3	-0.012688587869412515
1102	4	-0.009237548315510935
1102	5	-0.004438888154645326
1102	6	0.016366077847784766
1102	7	0.003296850954662034
1102	8	-0.032736742218048676
1102	9	-0.034979984777145035
1102	10-11	-2.0893708479974293E-4
1102	12-13	-0.011101137755836987
1102	14-15	-0.004509067653891918
1102	16-17	-0.026632730094206636
1102	18-19	-0.05095086730575815
1102	20-21	-0.05717390517371257
1102	22-23	-0.056979573811034356
1102	24-25	-0.04506048174242494
1102	26-27	-0.08133309079259021
1102	28-29	-0.07353852340279587
1102	30-31	-0.06485987779455371
1102	32-33	-0.10596070849193495
1102	34-35	-0.028657943333378455
1102	36-37	-0.004774520185769404
1102	38-39	-0.025114221171421036
1102	40-41	-0.00410110074757597
1102	42-43	0.004226379676971703
1102	44-45	-0.024586764770411662
1102	46-47	0.038112431061271934
1102	48-49	0.004358451317933998
1102	50-51	-0.030891048158331103
1102	52-53	-0.027212646698380638
1102	54-55	-0.06441595434402103
1102	56-57	-0.060403567530805446
1102	58-59	-0.07474411161508954
1102	60-61	-0.06109669121511985
1102	62-63	-0.07087758724903637
1102	64-65	-0.11613422587981148
1102	66-67	-0.11797663248951551
1102	68-69	-0.07983392473773421
1102	70-71	-0.09208095531959515
1102	72-73	-0.09616020284044424
1102	74-75	-0.08720558995270977
1102	76-77	-0.03372234026595322
1102	78-79	0.023850784675829573
1102	80-81	0.0012355062873226075
1102	82-83	-0.001083990232480403
1102	84-85	-0.05152551525573301
1102	86-87	-0.0429197553434193
1102	88-89	-0.03463254389662751
1102	90-91	-0.03478582615600345
1102	92-93	-0.08647273456730886
1102	94-95	-0.073485281016886
1102	96-97	-0.12665900990368328
1102	98-99	-0.10890828076983183
1102	100	0.011629829281794457
1103	1	0.1295535188485175
1103	2	0.04559947367435058
1103	3	0.060270280532378706
1103	4	0.007606349415503644
1103	5	-0.017851616941833015
1103	6	0.020740841071614113
1103	7	0.016069965016718868
1103	8	-0.025671454534148097
1103	9	-0.009969873631000326
1103	10-11	-0.006643309984866619
1103	12-13	-0.009187821198594293
1103	14-15	0.03449176617687755
1103	16-17	0.03021970146782138
1103	18-19	0.049453870416307666
1103	20-21	-0.01057812402090974
1103	22-23	-0.04638753615733293
1103	24-25	-0.04610493674073979
1103	26-27	-0.07750211334212764
1103	28-29	-0.06098228805851846
1103	30-31	-0.07043557099702724
1103	32-33	-0.05834239686903686
1103	34-35	-0.062323611418833025
1103	36-37	-0.054437650457778375
1103	38-39	0.002099702304768414
1103	40-41	-0.022855294848966423
1103	42-43	-0.0609309290665081
1103	44-45	-0.03427080239873703
1103	46-47	-0.040626291794779945
1103	48-49	-0.015718421228065438
1103	50-51	-0.03018582888887522
1103	52-53	-0.028283308579432287
1103	54-55	-0.014284080926948661
1103	56-57	-0.027488353901766516
1103	58-59	-0.05065672954000178
1103	60-61	-0.08719102690986347
1103	62-63	-0.13889526088676973
1103	64-65	-0.05417981968016505
1103	66-67	-0.06010331014838499
1103	68-69	-0.07722076439289083
1103	70-71	-0.06435766831183543
1103	72-73	-0.09927908108083017
1103	74-75	-0.13462856986268434
1103	76-77	-0.1338581968362007
1103	78-79	-0.047715557825235067
1103	80-81	-0.029767934685956732
1103	82-83	-0.002579196852188659
1103	84-85	-0.0015015346338884683
1103	86-87	4.933066785639539E-4
1103	88-89	0.04137045312045018
1103	90-91	0.024950917677529105
1103	92-93	-0.02174751539499198
1103	94-95	-0.04314548666614115
1103	96-97	-0.04505675546491261
1103	98-99	0.015402819393976586
1103	100	0.11312770561147545
1104	1	0.10997454759785086
1104	2	0.06614444130097752
1104	3	0.044973945749582356
1104	4	0.018506404408967114
1104	5	0.00680418817010775
1104	6	0.02309969142267221
1104	7	0.03046901851363515
1104	8	0.00605753567226941
1104	9	0.004135868648390328
1104	10-11	0.005525559465368701
1104	12-13	-0.0020239240799000413
1104	14-15	0.047352739778069974
1104	16-17	0.0015850179606431425
1104	18-19	0.018323109010900396
1104	20-21	-0.03524392569248391
1104	22-23	-0.03863653253595345
1104	24-25	-0.009231348832187791
1104	26-27	-0.005074213218883017
1104	28-29	-0.06820680057735728
1104	30-31	-0.045496186678605
1104	32-33	-0.05404626842287996
1104	34-35	-0.040541862230064396
1104	36-37	-0.0018490542875966298
1104	38-39	0.05466412323644221
1104	40-41	0.01574401108133827
1104	42-43	0.010510885873735276
1104	44-45	0.0017955745770876774
1104	46-47	0.05006126448441961
1104	48-49	0.10642488033230535
1104	50-51	0.02823493240745023
1104	52-53	0.01706082135351039
1104	54-55	-0.05849809866798239
1104	56-57	-0.025128197693817356
1104	58-59	-0.035975588440379624
1104	60-61	-0.01368847974031695
1104	62-63	0.01793435206591454
1104	64-65	0.005254911346696645
1104	66-67	0.0057243277070071485
1104	68-69	0.020257152019233615
1104	70-71	0.024035306038513227
1104	72-73	0.015196493378276443
1104	74-75	0.025743129242080443
1104	76-77	-0.05199051055647175
1104	78-79	0.08620996697246852
1104	80-81	0.06258709389480543
1104	82-83	0.13855690944797772
1104	84-85	0.1316406167480011
1104	86-87	0.1310379567343034
1104	88-89	0.11056869898090582
1104	90-91	0.09732781058040985
1104	92-93	0.06510064714222352
1104	94-95	0.06540001154364461
1104	96-97	0.02593139803780531
1104	98-99	0.07444778591175094
1104	100	0.005994744917913408
1105	1	0.09345228988699716
1105	2	0.051066090955288246
1105	3	-0.008823592455996732
1105	4	0.01608852623318313
1105	5	-0.0011741439772592344
1105	6	0.0010410513041634317
1105	7	0.018112941207299116
1105	8	-0.025266635869243714
1105	9	-0.004883923024586068
1105	10-11	0.005461940972011803
1105	12-13	-0.006621305696633328
1105	14-15	0.00651676704744375
1105	16-17	-0.031025243135324843
1105	18-19	-0.02579606492944464
1105	20-21	-0.05324264258977962
1105	22-23	-0.03358716313859844
1105	24-25	-0.040385236142967074
1105	26-27	-0.02391114503570435
1105	28-29	-0.031281529455633006
1105	30-31	-0.05011458674577085
1105	32-33	-0.09536032118437276
1105	34-35	-0.13270579081706302
1105	36-37	-0.10100709171266686
1105	38-39	-0.07770587604660051
1105	40-41	-0.024688624928593583
1105	42-43	-0.05064467242318216
1105	44-45	-0.08325451122534133
1105	46-47	-0.10939038153519931
1105	48-49	-0.09740396912567206
1105	50-51	-0.09550823244160256
1105	52-53	-0.07464030381212439
1105	54-55	-0.041248918373746335
1105	56-57	-0.0743422892889285
1105	58-59	-0.10679705562450437
1105	60-61	-0.03418542193949037
1105	62-63	-0.10153343802284809
1105	64-65	-0.1524187625853486
1105	66-67	-0.13079447739071526
1105	68-69	-0.08495911281849544
1105	70-71	-0.08555048870885429
1105	72-73	-0.025861130975449953
1105	74-75	-0.05778894632111786
1105	76-77	-0.06789708833104413
1105	78-79	-0.005787895428490231
1105	80-81	-0.04792681975071389
1105	82-83	-0.07595501049785724
1105	84-85	-0.0446746504680533
1105	86-87	-0.051305398750493225
1105	88-89	-0.07268368378094436
1105	90-91	-0.09583433815735987
1105	92-93	-0.09523874821697831
1105	94-95	-0.0709856656593999
1105	96-97	-0.10302156545274244
1105	98-99	-0.1166606277855422
1105	100	0.011486605974329933
1106	1	0.06578758734777779
1106	2	0.027457481923455873
1106	3	-0.00959426465704638
1106	4	-0.03145584887176511
1106	5	-0.019018255281594065
1106	6	-0.012927877204205629
1106	7	0.0019017069524878139
1106	8	-0.00949463944848361
1106	9	-0.013156398309476458
1106	10-11	-0.05062555375258171
1106	12-13	-0.05211116875842947
1106	14-15	0.007884826689796398
1106	16-17	0.0023899098843074285
1106	18-19	0.0056244479932345826
1106	20-21	-0.0031706333338661352
1106	22-23	-0.054150098684850434
1106	24-25	-0.016458374973737477
1106	26-27	-0.05422640973174708
1106	28-29	-0.042085576578429595
1106	30-31	-0.006518825399361106
1106	32-33	-0.05505196060148876
1106	34-35	-0.018555228803528223
1106	36-37	0.04186377543148723
1106	38-39	-0.03704296443320487
1106	40-41	-0.03223468188225809
1106	42-43	-0.01868200050666502
1106	44-45	-0.0076165600363395924
1106	46-47	-0.023859151108545973
1106	48-49	-0.02348446803463844
1106	50-51	-0.0306578134404063
1106	52-53	-0.05512867230413576
1106	54-55	-0.021963220941088935
1106	56-57	-0.013569010784891589
1106	58-59	0.011021339718396916
1106	60-61	0.01727239839116379
1106	62-63	-0.07074088533725842
1106	64-65	-0.01607923912677478
1106	66-67	-0.07589377603772363
1106	68-69	0.012209468443195703
1106	70-71	-0.030765604175044814
1106	72-73	0.006391019306768442
1106	74-75	-0.0070396761384827755
1106	76-77	-0.03859352791590709
1106	78-79	0.005245126597962724
1106	80-81	0.03442608702484762
1106	82-83	0.03392016811631038
1106	84-85	0.09501354140331131
1106	86-87	0.11465207386965659
1106	88-89	0.06672951213398193
1106	90-91	0.09754555781107399
1106	92-93	0.0583976223568996
1106	94-95	0.051647841536784256
1106	96-97	0.06863658995489885
1106	98-99	0.07777122496440825
1106	100	0.12007547705848154
1107	1	0.11492357498133998
1107	2	0.03826670422649414
1107	3	0.02622035582715654
1107	4	0.012941657057744749
1107	5	0.03717298481485898
1107	6	0.040225968339541396
1107	7	0.017742135578117768
1107	8	0.01488851053927931
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2316	7	-0.009562520604980307
2316	8	0.0019734808923033142
2316	9	-0.03724524093659909
2316	10-11	-0.028343368285291604
2316	12-13	-0.01784912132576011
2316	14-15	0.023018736648978688
2316	16-17	0.03608874981266297
2316	18-19	0.01524124559088591
2316	20-21	-0.004444128901340605
2316	22-23	-0.028880332423121047
2316	24-25	-0.016694665955299115
2316	26-27	-0.010777340537288183
2316	28-29	0.020501859512663145
2316	30-31	-0.2400416709517117
2316	32-33	-0.03873436921702478
2316	34-35	-0.1357517498066514
2316	36-37	-0.4460353289182848
2316	38-39	-0.2785797612959655
2316	40-41	-0.17210105354614313
2316	42-43	-0.26273504710756157
2316	44-45	-0.1428484475844698
2316	46-47	-0.3063967897108242
2316	48-49	-0.27883363770637715
2316	50-51	-0.2533699312276454
2316	52-53	-0.2685681077775257
2316	54-55	-0.1114965031710966
2316	56-57	-0.7742933849279652
2316	58-59	-0.6479768931608234
2316	60-61	-0.3383368932440831
2316	62-63	-0.11056001619195399
2316	64-65	-0.03185348296351975
2316	66-67	0.02207875615474819
2316	68-69	-0.1154930806797978
2316	70-71	-0.1425065583970806
2316	72-73	-0.08253883804088247
2316	74-75	0.025552835736363022
2316	76-77	-0.022284630092201496
2316	78-79	-0.07533302494410776
2316	80-81	-0.0796051809506011
2316	82-83	-0.25055793120547776
2316	84-85	-0.053980900020874856
2316	86-87	-0.10012294851409109
2316	88-89	-0.13430396581222936
2316	90-91	-0.11553366480183769
2316	92-93	-0.15063303315719168
2316	94-95	-0.08830454808109067
2316	96-97	-0.2729172569124074
2316	98-99	-0.056714349370714956
2316	100	-0.3143480619589276
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	12.0
13	95.0
14	405.0
15	1334.0
16	3461.0
17	7539.0
18	13832.0
19	22026.0
20	31706.0
21	45539.0
22	59951.0
23	64186.0
24	60976.0
25	59513.0
26	64391.0
27	75134.0
28	92075.0
29	112806.0
30	139094.0
31	172118.0
32	216104.0
33	282184.0
34	407148.0
35	620017.0
36	1111197.0
37	2303561.0
38	2919164.0
39	694542.0
40	234.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.42123910954223	19.179628722788586	13.513526819266191	23.885605348402994
2	15.912279509614164	19.96335602679375	40.06092506774062	24.063439395851464
3	18.866314675108566	25.10104743618944	30.027617510115185	26.005020378586817
4	12.768618289957548	16.66401537332611	38.207203793645874	32.36016254307047
5	13.424469126402661	37.413560054852255	34.84176324283394	14.320207575911148
6	31.29884543847942	36.94806670363819	17.651469200437102	14.10161865744529
7	27.43765019686665	32.27824932781635	22.065535649402115	18.218564825914896
8	26.000203959762935	35.17172336918565	19.968255760949972	18.859816910101447
9	26.065976314931333	15.7760235756746	20.60788029783579	37.550119811558275
10-11	25.104814874330966	25.92496813919042	28.06750091998267	20.902716066495948
12-13	25.597734402383548	23.61702223156543	28.180799808608086	22.604443557442938
14-15	22.937293479617253	25.11255496228492	25.977505238734473	25.972646319363356
16-17	22.34363523044435	27.89560473799518	26.76124508746507	22.999514944095402
18-19	22.00587050903997	27.168773455458066	28.841222933470647	21.98413310203132
20-21	23.002081767189036	26.372025908941264	28.719660988430242	21.906231335439454
22-23	22.85540760607054	26.220190954710404	28.431089749528056	22.493311689691
24-25	22.50688310141585	26.317298319152744	28.77583139733874	22.399987182092673
26-27	22.516561199442787	26.50081572326358	28.426245167285245	22.556377910008386
28-29	22.24521342576559	26.672467388555543	28.60999027326463	22.472328912414234
30-31	22.791746709645746	26.3864717461919	28.567414259871036	22.254367284291316
32-33	22.63022054270813	26.561779508236853	28.285134473352592	22.522865475702428
34-35	22.814257885523666	26.60319890504958	28.447373183266556	22.1351700261602
36-37	22.72324524744212	26.46721185132787	28.275042322703587	22.53450057852642
38-39	22.651186563402877	26.601331316162323	28.39819786677067	22.349284253664127
40-41	22.763908642098908	26.22040474420849	28.52970992112038	22.48597669257222
42-43	22.564402155744542	26.30976220409258	28.32476652598908	22.8010691141738
44-45	22.598528604571992	26.712774140859207	28.18197233457605	22.50672491999275
46-47	22.605955630742	26.576757137797358	27.84015246341211	22.97713476804853
48-49	22.80572149757197	26.605277936558636	27.81088614541784	22.778114420451555
50-51	22.762190585067916	26.747956370845312	27.602302543127276	22.8875505009595
52-53	22.62893530753713	26.954737912175464	27.462358404452203	22.953968375835203
54-55	22.516344754271262	26.851796565952306	27.574345771408098	23.05751290836833
56-57	22.817664636203723	26.82207367128107	27.352666179731326	23.00759551278388
58-59	22.497599628153083	26.87695717659383	27.660675909848838	22.964767285404253
60-61	22.638549354360897	27.01661067771947	27.428162971911096	22.916676996008533
62-63	22.600426929891213	26.8290484555145	27.390442317258252	23.18008229733604
64-65	22.345901188624925	26.76109050287913	27.478854033385545	23.4141542751104
66-67	22.510120984179956	26.732023206775168	27.36946162872364	23.388394180321235
68-69	22.37399491805433	26.76607945834889	27.456154570645737	23.403771052951043
70-71	22.417846066326472	26.547649398959578	27.42322039714465	23.6112841375693
72-73	22.517581250785483	26.69776031378404	27.32422611390371	23.460432321526767
74-75	22.579248710904896	26.87605145440493	27.11266392667409	23.432035908016086
76-77	22.44826328014267	26.82341245205296	27.030224153613	23.698100114191377
78-79	22.36617209905047	26.807729682506704	27.08760941136167	23.738488807081158
80-81	22.239732116878415	27.083605702223757	27.07205073746405	23.604611443433775
82-83	22.580330504376313	26.755428076724314	27.32392119809689	23.340320220802486
84-85	22.65447416168917	26.83109777689981	26.936742725427777	23.577685335983244
86-87	22.162500255742515	27.10233395838892	27.125418634840187	23.609747151028376
88-89	22.35894351007764	26.706970645849555	27.091091199548067	23.84299464452474
90-91	22.379949407773253	26.760233772850412	27.059602515593024	23.80021430378331
92-93	22.408557692583702	26.768201236880103	27.0337867794902	23.789454291045995
94-95	22.005780873868005	26.75513498665637	27.193870129658652	24.045214009816974
96-97	22.092195055884925	26.72214058664362	27.1133702898511	24.072294067620355
98-99	21.879648610819853	26.83696815925685	27.00873483465337	24.274648395269928
100	22.088490243376196	26.676570522646564	26.853395270011077	24.381543963966166
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1152.0
1	847.0
2	976.5
3	1364.0
4	1030.5
5	894.5
6	1420.5
7	2701.5
8	4472.0
9	5655.5
10	5388.5
11	4807.5
12	5146.5
13	5956.5
14	6819.0
15	7736.0
16	8664.0
17	9783.0
18	10911.5
19	12009.0
20	13351.5
21	14813.0
22	16886.5
23	19928.0
24	23843.5
25	29051.0
26	36203.0
27	45667.5
28	57220.5
29	71803.5
30	89409.5
31	107739.0
32	130130.0
33	153129.0
34	174360.5
35	195801.0
36	220819.5
37	251188.0
38	269618.5
39	277373.5
40	281630.0
41	283596.5
42	292981.5
43	309211.5
44	326119.5
45	350353.5
46	372344.5
47	388269.0
48	403229.0
49	406880.5
50	398961.0
51	392774.0
52	384903.5
53	370866.0
54	356259.5
55	326736.5
56	292370.5
57	254859.5
58	218750.5
59	192883.0
60	161773.0
61	129108.5
62	102038.0
63	79473.0
64	58389.0
65	40389.0
66	28591.5
67	21010.0
68	15750.0
69	11820.0
70	9052.5
71	6910.0
72	5110.5
73	3722.0
74	2739.5
75	1906.0
76	1255.5
77	753.5
78	448.5
79	272.0
80	149.5
81	78.5
82	48.0
83	33.0
84	13.5
85	7.5
86	8.0
87	6.0
88	4.0
89	3.0
90	2.5
91	1.5
92	0.5
93	0.0
94	1.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.35042572048799486
2	5.636539052291113E-4
3	0.0020145408094299714
4	0.0012212501279964076
5	0.004488355171268848
6	0.0
7	4.1752141128082314E-5
8	2.7138891733253503E-4
9	0.0031627246904522353
10-11	3.6533123487072026E-5
12-13	5.219017641010289E-5
14-15	2.8704597025556593E-4
16-17	8.350428225616463E-5
18-19	9.133280871768006E-4
20-21	2.6095088205051447E-4
22-23	1.3047544102525723E-4
24-25	0.0
26-27	0.0020719500034810847
28-29	0.011267859086941213
30-31	0.003522836907681945
32-33	0.014467116900880522
34-35	0.011487057827863646
36-37	0.013240647755243104
38-39	0.013099734278935826
40-41	0.01444102181267547
42-43	0.006685561598134181
44-45	0.0012682212867655002
46-47	0.006325449380904471
48-49	0.013376342213909372
50-51	0.003454989678348811
52-53	0.0057670144933163695
54-55	0.005375588170240598
56-57	2.0354168799940127E-4
58-59	6.315011345622449E-4
60-61	3.4967418194768935E-4
62-63	8.924520166127595E-4
64-65	3.4445516430667907E-4
66-67	0.0013308494984576238
68-69	0.0016492095745592513
70-71	8.82013981330739E-4
72-73	0.0026616989969152475
74-75	0.007922468779053619
76-77	0.003929920283680747
78-79	0.0016753046627643027
80-81	5.166827464600187E-4
82-83	2.0876070564041155E-4
84-85	0.0015082960982519735
86-87	0.0041647760775262105
88-89	0.0015813623452261177
90-91	2.2441775856344242E-4
92-93	0.0020301978623530025
94-95	0.0032096958492213276
96-97	0.0017692469803024878
98-99	0.0020876070564041158
100	0.0025051284676849386
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	9580347.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.32493102368799
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.37158148035802	42.79392428940784
2	15.502108023427793	18.083187623763724
3	5.145945152416284	9.004106881990841
4	2.23821610700979	5.221752002298137
5	1.156955396036019	3.3739671835642215
6	0.678096513438634	2.3729959424226985
7	0.4097341987945523	1.672840321791683
8	0.28516486213602354	1.3305776731570453
9	0.21245329469178728	1.1152191382788377
>10	0.9310036207360292	9.378822820414554
>50	0.04617643437435076	1.8206572640606586
>100	0.020922802530202334	2.079055349862259
>500	0.0012088569321514636	0.4728772549537733
>1k	3.420450951586503E-4	0.33430471799142114
>5k	2.280300574558938E-5	0.07889463529332959
>10k+	6.840901723676814E-5	0.8668169007489475
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27197	0.28388324556511363	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24058	0.25111825281485106	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13920	0.14529745112572645	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	4.1752141128082314E-5	0.0	0.0	0.0	0.0
7	4.1752141128082314E-5	0.0	0.0	0.0	0.0
8	4.1752141128082314E-5	0.0	0.0	0.0	1.0438035282020578E-5
9	4.1752141128082314E-5	0.0	0.0	1.0438035282020578E-5	3.131410584606173E-5
10-11	4.69711587690926E-5	0.0	0.0	6.262821169212348E-5	3.131410584606173E-5
12-13	5.219017641010289E-5	0.0	0.0	7.306624697414405E-5	7.306624697414404E-5
14-15	5.219017641010289E-5	5.219017641010289E-6	0.0	7.306624697414405E-5	1.1481838810222637E-4
16-17	8.350428225616463E-5	1.0438035282020578E-5	0.0	7.828526461515435E-5	1.5135151158929838E-4
18-19	8.350428225616463E-5	1.0438035282020578E-5	0.0	1.0438035282020578E-4	1.5657052923030867E-4
20-21	8.350428225616463E-5	1.0438035282020578E-5	0.0	1.8788463507637038E-4	1.6178954687131897E-4
22-23	8.350428225616463E-5	1.0438035282020578E-5	0.0	3.96645340716782E-4	1.8266561743536012E-4
24-25	8.350428225616463E-5	1.565705292303087E-5	0.0	8.924520166127594E-4	2.0876070564041155E-4
26-27	8.350428225616463E-5	2.0876070564041157E-5	0.0	0.001863189297840673	2.452938291274836E-4
28-29	8.350428225616463E-5	2.0876070564041157E-5	0.0	0.004968504794241795	2.5051284676849385E-4
30-31	8.350428225616463E-5	2.0876070564041157E-5	0.0	0.01661735216897676	2.557318644095042E-4
32-33	8.350428225616463E-5	2.0876070564041157E-5	0.0	0.04351095007310278	2.766079349735453E-4
34-35	8.872329989717491E-5	2.0876070564041157E-5	0.0	0.08654696954087363	3.1314105846061733E-4
36-37	9.39423175381852E-5	2.0876070564041157E-5	0.0	0.15275542733473016	3.1314105846061733E-4
38-39	9.39423175381852E-5	2.6095088205051444E-5	0.0	0.2477833005422455	3.2879811138364823E-4
40-41	1.0438035282020578E-4	3.131410584606173E-5	0.0	0.35180876016286255	3.5489319958869963E-4
42-43	1.0438035282020578E-4	3.131410584606173E-5	0.0	0.45634046449465765	3.653312348707202E-4
44-45	1.0438035282020578E-4	3.131410584606173E-5	0.0	0.5835018293178733	3.862073054347614E-4
46-47	1.0438035282020578E-4	3.131410584606173E-5	0.0	0.7333867969500478	4.279594465628437E-4
48-49	1.0438035282020578E-4	3.131410584606173E-5	0.0	0.8903957236622013	4.5927355240890546E-4
50-51	1.0959937046121607E-4	3.131410584606173E-5	0.0	1.0502229199005004	4.5927355240890546E-4
52-53	1.1481838810222637E-4	3.131410584606173E-5	0.0	1.2106763982557207	5.010256935369878E-4
54-55	1.1481838810222637E-4	3.131410584606173E-5	0.0	1.3901062247536546	5.427778346650701E-4
56-57	1.1481838810222637E-4	4.1752141128082314E-5	0.0	1.5976926514248389	5.427778346650701E-4
58-59	1.1481838810222637E-4	4.1752141128082314E-5	0.0	1.8262647480305254	5.479968523060803E-4
60-61	1.2003740574323665E-4	4.1752141128082314E-5	0.0	2.0638396500669547	5.636539052291112E-4
62-63	1.461324939482881E-4	4.1752141128082314E-5	0.0	2.30066823258072	6.158440816392141E-4
64-65	1.461324939482881E-4	4.1752141128082314E-5	0.0	2.5452731513795896	6.158440816392141E-4
66-67	1.461324939482881E-4	4.1752141128082314E-5	0.0	2.8213800606595982	6.315011345622449E-4
68-69	1.461324939482881E-4	4.1752141128082314E-5	0.0	3.123983922503016	6.367201522032552E-4
70-71	1.461324939482881E-4	4.1752141128082314E-5	0.0	3.4474012266987826	6.784722933313376E-4
72-73	1.7222758215333953E-4	4.1752141128082314E-5	0.0	3.7752442578541254	7.724146108695227E-4
74-75	1.7744659979434982E-4	4.1752141128082314E-5	0.0	4.1025340731395215	7.776336285105331E-4
76-77	1.826656174353601E-4	4.1752141128082314E-5	0.0	4.4587476841913976	7.880716637925537E-4
78-79	1.878846350763704E-4	4.1752141128082314E-5	0.0	4.85095164089568	7.93290681433564E-4
80-81	1.878846350763704E-4	4.1752141128082314E-5	0.0	5.265727848897331	8.298238049206359E-4
82-83	1.878846350763704E-4	4.1752141128082314E-5	0.0	5.692586082737922	8.924520166127594E-4
84-85	1.931036527173807E-4	4.1752141128082314E-5	0.0	6.116683456246418	8.976710342537697E-4
86-87	2.0354168799940127E-4	4.1752141128082314E-5	0.0	6.560628753843676	9.081090695357904E-4
88	2.1919874092243214E-4	4.1752141128082314E-5	0.0	6.9143424554455075	9.185471048178109E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14865	0.0	54.117786	1
GTATCAA	27085	0.0	41.400818	1
CAACGCA	32685	0.0	33.201637	5
ATCAACG	32725	0.0	33.089767	3
TATCAAC	34565	0.0	32.12903	2
TCAACGC	34650	0.0	31.427284	4
AACGCAG	35460	0.0	31.025879	6
GTGGTAT	6460	0.0	27.300146	1
ACGCAGA	39925	0.0	27.214888	7
CGCAGAG	40925	0.0	26.480991	8
GTACATG	30965	0.0	24.609127	1
GTACTAG	4005	0.0	24.372143	1
TACATGG	31460	0.0	23.603088	2
GCAGAGT	45595	0.0	23.387344	9
ACATGGG	31535	0.0	22.445175	3
TGGTATC	7200	0.0	22.323591	2
GAGTACT	28125	0.0	21.437883	12-13
CATGGGG	23315	0.0	21.1458	4
CAGAGTA	43945	0.0	20.5803	10-11
AGAGTAC	41370	0.0	20.509508	10-11
>>END_MODULE