FastQCFastQC Report
Thu 26 May 2016
ERR580245_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580245_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6187213
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140210.22661253136105058No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112860.1824084608045658No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88300.049.1556131
GTATCAA154950.040.7279971
CAACGCA181650.033.717465
ATCAACG182000.033.5748673
TATCAAC191450.032.6602442
TCAACGC194850.031.5059194
AACGCAG199350.031.1340586
ACGCAGA217100.028.2215567
GTGGTAT35650.027.3050461
CGCAGAG224750.027.135528
GTACATG218350.026.8777871
TACATGG221600.025.86172
GTACTAG28150.025.224971
ACATGGG222750.025.0481323
GCAGAGT253350.023.4631049
CATGGGG184650.023.0891194
TAGTACT36700.023.0545024
TGGTATC42550.022.3190122
CTAGTAC37000.022.1049653
GAGTACT133500.021.8601212-13
Produced by FastQC (version 0.11.5)