FastQCFastQC Report
Thu 26 May 2016
ERR580245_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580245_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6187213
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130470.2108703870385584No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109920.1776567252493166No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85250.050.5133741
GTATCAA152500.041.154441
CAACGCA179500.033.6723185
ATCAACG182700.033.006973
TATCAAC191950.032.524852
AACGCAG195800.031.4674766
TCAACGC195650.030.9894144
GTACTAG31050.028.6157741
ACGCAGA220050.027.3803237
GTACATG212800.026.444041
CGCAGAG228500.026.1824688
TACATGG215450.025.7914832
GTGGTAT36900.025.4805581
ACATGGG217600.024.3218733
GCAGAGT257350.022.80819
TAGGCAT41550.022.3970415
CATGGGG180450.021.9049664
TGGTATC40700.021.484432
ATGGGGG130450.021.2571185
GAGTACT139600.020.61910212-13
Produced by FastQC (version 0.11.5)