FastQCFastQC Report
Thu 26 May 2016
ERR580244_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580244_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8321716
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188900.22699645121270662No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143950.17298114956097996No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88040.105795487373037No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA119200.044.2298361
GTATCAA211100.040.4588551
CAACGCA234650.034.4508135
ATCAACG241100.033.5141033
TATCAAC264350.032.167562
GTACTAG71000.031.5970121
AACGCAG273750.030.3724776
GCATCGT69950.028.892128
GTACATG316600.028.7742961
TCAACGC287250.028.5541864
ACGCAGA285800.028.269297
TACATGG322200.027.8809052
TAGGCAT85550.027.704785
CGCAGAG293250.027.4868358
GGGCACC80250.027.40774794
ACATGGG316550.027.3980873
TAGTACT96150.026.4136124
CTGTGCG95800.025.9041739
CATGGGG268700.025.5545674
TCTAACG34000.025.3146082
Produced by FastQC (version 0.11.5)