FastQCFastQC Report
Thu 26 May 2016
ERR580244_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580244_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8321716
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156470.1880261234581906No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125000.1502094039258249No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA121500.042.9472661
GTATCAA205500.038.454281
CAACGCA227350.032.952695
ATCAACG236000.031.7255463
GTACTAG67150.030.3831351
TATCAAC262350.029.9196432
AACGCAG267900.028.963676
GGGCACC80350.028.54920894
TCAACGC279100.027.1629684
TAGTACT97350.026.988494
GTACATG308850.026.8345011
CTGTGCG94750.026.735699
ACGCAGA285650.026.2425297
TACATGG312800.025.9505522
TAGGCAT87400.025.9198885
CTAACGC33050.025.740233
TCTAACG33950.025.6125562
TAACGCC33050.025.5978664
CGCAGAG293550.025.4882628
ACATGGG308350.025.4552783
Produced by FastQC (version 0.11.5)