FastQCFastQC Report
Thu 26 May 2016
ERR580243_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580243_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10143349
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT256140.25252014891728564No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202700.19983537981390567No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124970.12320388463415782No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA149500.045.1371461
GTATCAA266400.040.1049041
CAACGCA292750.034.5004125
ATCAACG301650.033.470053
TATCAAC331600.031.8514562
GTACTAG77300.031.6334971
AACGCAG336150.030.7756146
TCAACGC348600.029.230894
GTACATG377850.028.5618691
GCATCGT76050.028.2441338
ACGCAGA359500.028.0571927
TAGGCAT94850.027.9098835
TACATGG379750.027.8871862
GGGCACC88850.027.71879894
CTGTGCG107300.027.2896189
CGCAGAG369550.027.2813178
GTGGTAT49150.027.2674621
TAGTACT110300.027.2418044
ACATGGG377750.026.814453
CTAGGCA107100.025.4214084
Produced by FastQC (version 0.11.5)