FastQCFastQC Report
Thu 26 May 2016
ERR580243_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580243_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10143349
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT215160.21211929117296466No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177990.17547458930970433No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116230.11458740106448079No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA151100.043.774691
GTATCAA265100.039.8993871
CAACGCA297500.033.903475
ATCAACG307750.032.8509563
TATCAAC338050.031.1315142
AACGCAG343200.030.209136
GTACTAG76500.029.6810421
TCAACGC353350.028.8905684
GGGCACC90600.028.27675894
GTACATG370100.027.6650981
ACGCAGA364850.027.6307647
CGCAGAG373450.026.969438
TACATGG375500.026.7498682
CTGTGCG105750.026.6656999
TAGTACT109600.026.4591564
ACATGGG373250.025.8772223
GTGGTAT50800.025.6335931
TAGGCAT94700.025.3117145
CTAGTAC123400.024.4145373
CATGGGG306300.024.3211234
Produced by FastQC (version 0.11.5)