FastQCFastQC Report
Thu 26 May 2016
ERR580242_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580242_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7585230
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204460.2695501652553713No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161630.21308516683080145No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99520.13120234982986673No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA112350.047.7237661
GTATCAA205150.041.656841
CAACGCA240350.034.1648985
ATCAACG245200.033.5303653
TATCAAC260400.032.475832
AACGCAG269650.031.238376
TCAACGC272950.030.2765184
GTGGTAT39800.030.2524011
GTACTAG51600.030.0792641
ACGCAGA295750.027.9086677
GTACATG296900.027.8174481
TACATGG300650.027.0017012
CGCAGAG307100.026.8453488
TAGGCAT65150.025.9877155
CTAACGC24700.025.8975853
ACATGGG304100.025.6285113
TAACGCC25200.025.1970984
TAGTACT68600.025.0942524
TCTAACG25600.024.9867922
GCATCGT56100.024.9673638
Produced by FastQC (version 0.11.5)