FastQCFastQC Report
Thu 26 May 2016
ERR580242_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580242_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7585230
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179660.2368550459247775No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143590.18930210422096627No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94610.12472924354304353No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA112150.048.9168321
GTATCAA196350.040.102431
CAACGCA232450.032.453435
ATCAACG238100.031.7032783
TATCAAC252700.031.0072122
GTACTAG46550.030.0942361
AACGCAG264400.029.0987116
TCAACGC264600.028.8123744
GTACATG288600.027.4141351
TACATGG295600.026.379972
ACGCAGA288250.026.2182247
ACATGGG294200.025.4342173
CGCAGAG298100.025.3519048
GTGGTAT40200.024.6742041
CTAACGC23100.023.80633
TAGTACT68000.023.7775754
TAACGCC23800.023.5010914
TAGGCAT63700.023.464045
CATGGGG243350.023.3127754
TCTAACG24050.023.2575632
Produced by FastQC (version 0.11.5)