FastQCFastQC Report
Thu 26 May 2016
ERR580241_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580241_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8359011
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187910.22479932135512204No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149240.17853786769750632No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89010.10648388906295254No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA128400.043.2926981
GTATCAA223250.039.076411
CAACGCA234650.035.0693475
GTACTAG72350.034.385821
ATCAACG246900.033.390713
GGGCACC84250.032.411894
TATCAAC277100.031.3476922
AACGCAG275650.030.607436
GCATCGT76100.030.1397698
TAGGCAT93200.029.5659525
TAGTACT106850.028.607864
GTGGTAT39150.028.5895561
CTGTGCG105400.028.5390119
ACGCAGA289600.028.3868267
GTACATG325300.028.2923451
TCAACGC297650.028.2019374
CTAGGCA102650.027.7167974
CGCAGAG297400.027.6093838
TACATGG329750.027.4264552
TCTAACG35200.027.2560372
Produced by FastQC (version 0.11.5)