FastQCFastQC Report
Thu 26 May 2016
ERR580241_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580241_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8359011
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162230.19407798362748896No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137020.1639189133738429No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86210.10313421049451903No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA125150.042.8588331
GTATCAA214400.038.765291
GGGCACC87050.034.07376594
CAACGCA234800.033.5284845
GTACTAG72700.032.654461
ATCAACG245600.032.149983
TATCAAC270600.030.483312
TAGTACT108350.029.7138444
CTGTGCG107100.029.5331529
AACGCAG277700.029.3291246
TAGGCAT92750.027.617215
TCAACGC291950.027.4481534
CTAGTAC121350.027.3828983
GTACATG319900.027.2152481
ACGCAGA291700.026.9870117
GTGGTAT36450.026.9546871
GTACTGT122350.026.8119186
TACATGG323650.026.474262
ACTGTGC131200.026.3287328
CGCAGAG299700.026.2352758
Produced by FastQC (version 0.11.5)