FastQCFastQC Report
Thu 26 May 2016
ERR580240_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580240_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6618796
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157830.23845726624600605No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129010.19491460380407555No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74500.11255823566703067No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99850.049.5926171
GTATCAA179850.040.8681761
CAACGCA204900.034.0922625
ATCAACG207800.033.7954143
TATCAAC223150.032.487952
GTACTAG38350.031.7593081
AACGCAG226900.031.3704416
TCAACGC233150.030.102334
ACGCAGA246450.028.1778477
GCATCGT40150.027.9684248
CGCAGAG252150.027.4100198
GTACATG253350.026.2090021
GTGGTAT34750.026.1179561
TAGGCAT55200.025.1224445
TACATGG259950.025.0854242
TAGTACT56600.024.7500174
ACATGGG255800.024.5320243
CTGTGCG55150.024.3694139
GCAGAGT278800.024.3219369
CTAGGCA60800.023.8907954
Produced by FastQC (version 0.11.5)