FastQCFastQC Report
Thu 26 May 2016
ERR580240_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580240_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6618796
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139740.21112601143772977No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118440.17894493197856529No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70540.1065752744154677No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99450.046.4738041
GTATCAA181000.041.1727751
CAACGCA208700.034.254575
ATCAACG211900.033.782153
TATCAAC227200.032.5120052
GTACTAG38700.031.587981
AACGCAG233250.031.272946
TCAACGC235850.030.5708734
GTGGTAT37600.029.5109791
ACGCAGA251150.028.3142097
CGCAGAG256050.027.6803728
GTACATG249450.025.5962141
TAGGCAT52750.025.037775
TACATGG253050.024.8614042
CTGTGCG52300.024.7106259
GCAGAGT286650.024.3131899
ACATGGG248250.024.234783
TAGTACT55850.023.9850184
CTAGTAC60000.023.3444353
TGGTATC45950.023.0235822
Produced by FastQC (version 0.11.5)