FastQCFastQC Report
Thu 26 May 2016
ERR580239_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580239_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13339321
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT248080.18597648261107144No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161290.12091320090430391No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA309700.041.5201031
GGTATCA171300.040.5805781
CAACGCA337050.036.471925
ATCAACG351900.034.977113
TATCAAC388850.032.803252
AACGCAG398750.031.8622176
GTACTAG98750.030.9583191
TCAACGC423250.029.5021724
ACGCAGA420900.029.236567
CGCAGAG436350.028.1677868
GTACATG494250.027.3965641
TACATGG499350.026.5427072
CTAACGC48750.026.5312443
GCATCGT102150.026.4572968
TAACGCC50450.026.1957954
TCTAACG50900.025.7807622
ACATGGG499550.025.6464713
GGGCACC118750.025.13085294
TAGGCAT132850.024.9913945
GCAGAGT486300.024.7717729
Produced by FastQC (version 0.11.5)