FastQCFastQC Report
Thu 26 May 2016
ERR580239_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580239_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13339321
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219670.16467854698151427No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146830.1100730689365673No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA305650.041.86511
GGTATCA168600.038.8402441
CAACGCA342850.035.477865
ATCAACG353750.034.3992653
TATCAAC393300.032.2070962
AACGCAG405700.031.1624416
GTACTAG101800.031.0320111
TCAACGC420500.029.4518034
ACGCAGA433900.028.0756347
CGCAGAG448800.027.0808018
GTACATG503800.026.5748161
TACATGG505200.026.040922
CTGTGCG135950.025.8244159
TAGTACT133950.025.4736484
TAGGCAT135200.025.4119455
ACATGGG504700.024.9676113
GGGCACC122550.024.89385294
CTAGGCA151500.024.2910544
GCAGAGT501700.023.8601939
CTAGTAC155300.023.1527983
Produced by FastQC (version 0.11.5)