FastQCFastQC Report
Thu 26 May 2016
ERR580238_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580238_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5813232
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79200.13624090695158905No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66690.1147210364217358No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100700.043.6979641
GTATCAA178000.040.3041151
CAACGCA190750.035.586385
TATCAAC214500.033.3359642
ATCAACG203900.033.178063
GTGGTAT29300.032.7323651
GTACTAG56500.032.5344281
TACCCCG4150.030.5841835
AACGCAG234850.030.1938976
TAGGCAT77500.028.8726395
GCATCGT59700.028.4931058
ACGCAGA242800.027.810817
CGCAGAG243750.027.4710838
GGGCACC58450.026.53191494
CTAGGCA87150.026.3242534
TCAACGC262850.026.2198164
AAGCTAA48150.025.3788729
GCAGAGT263550.024.9309839
CTGTGCG72550.024.7469229
CGTTAAC18300.024.148581
Produced by FastQC (version 0.11.5)