FastQCFastQC Report
Thu 26 May 2016
ERR580238_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580238_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5813232
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70600.12144707109573469No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61720.10617157546782925No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102650.046.4556921
GTATCAA180100.039.8085751
TACCCCG4800.034.268435
CAACGCA196400.033.931435
ATCAACG207650.032.2068183
TATCAAC224250.031.8553452
GTACTAG53700.030.6547551
GTGGTAT28300.029.5007841
AACGCAG239800.028.8471986
GGGCACC58700.027.22149394
TAGGCAT75850.026.7047865
ACGCAGA251400.026.338487
TAGTACT70150.026.1281154
AAGCTAA45850.025.8293619
CGCAGAG255350.025.820858
CTGTGCG69700.025.5549
TCAACGC266450.025.36384
CTAGGCA84100.024.7559094
CGTTAAC22300.024.535781
GCAGAGT274600.023.6344559
Produced by FastQC (version 0.11.5)