FastQCFastQC Report
Thu 26 May 2016
ERR580237_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580237_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5514808
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164030.29743555895327634No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125000.22666246948216512No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79400.14397600061507126No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76500.049.061721
GTATCAA140850.042.007331
CAACGCA164150.035.1226965
ATCAACG168350.034.306683
TATCAAC176500.033.362682
AACGCAG183850.032.213096
TCAACGC182700.031.6878834
GTACTAG28950.030.7053851
GTACATG185300.028.732431
ACGCAGA202350.028.4544667
CGCAGAG209200.027.5452278
TACATGG192200.027.040222
GCATCGT29300.026.9500278
CTGTGCG43250.026.7334339
ACATGGG188250.026.4828263
TAGTACT43650.025.9660874
CTAGTAC45550.024.884113
GTGGTAT31750.024.442271
CATGGGG151850.024.4053554
GCAGAGT235350.023.9847159
Produced by FastQC (version 0.11.5)