FastQCFastQC Report
Thu 26 May 2016
ERR580237_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580237_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5514808
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144690.26236634167499573No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116830.21184781047681078No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81050.14696794521223586No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79950.047.9201161
GTATCAA137350.038.845971
GTACTAG32800.030.8136651
CAACGCA167950.030.7543035
ATCAACG170300.030.2195283
TATCAAC183200.028.9657122
AACGCAG187350.028.371956
GTACATG183700.028.0210061
TCAACGC185350.027.9683724
TACATGG184100.027.7007622
ACATGGG184500.026.5945283
CTGTGCG41600.025.9849979
CATGGGG150950.025.1260764
ACGCAGA205850.025.0458667
TAGTACT43850.024.758634
CTAGTAC44800.024.0240173
CGCAGAG213950.024.0102168
TAGGCAT43850.023.9014055
ATGGGGG110600.023.159525
CTAGGCA47400.023.0034984
Produced by FastQC (version 0.11.5)