FastQCFastQC Report
Thu 26 May 2016
ERR580236_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580236_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8090888
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183490.22678598443088074No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134100.16574200508028292No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC115660.1429509344338965No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86320.10668791855727085No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT21050.046.2478031
GGGCACC99100.042.63894394
GTATCAA247900.038.75831
GGTATCA142600.038.0261761
CAACGCA241500.036.8208275
ATCAACG255900.034.73523
GTACTAG109000.033.913211
TCAAGAC12150.032.9018943
TATTCTC38950.032.7175645
TATCAAC300000.032.0284652
TACCCCG5000.031.976295
TAGGCAT131550.031.7782555
ACGCAGA293550.030.244297
AACGCAG310600.030.2190536
AGGGCTA95100.030.1625775
CGCAGAG294600.030.072318
CTGTGCG150550.029.8445669
GCATCGT117450.029.611948
TAGTACT153600.029.42354
ACTGTGC154500.029.2640728
Produced by FastQC (version 0.11.5)