FastQCFastQC Report
Thu 26 May 2016
ERR580236_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580236_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8090888
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151310.18701284704472487No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC119700.14794420587703105No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115250.14244419153991503No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC100300.043.2098194
GGTATCA146250.039.311581
GTGGTAT19300.037.5105441
GTATCAA246400.036.860091
TACCCCG5000.033.8409045
GTACTAG114900.033.753891
CAACGCA248700.033.7532125
TATTCTC40650.033.0685275
ATCAACG261250.032.168143
TATTAAG18650.032.008192
TAGGCAT136850.030.77325
AGGGCTA98850.030.6684955
TATCAAC302150.029.9774992
TTAAGGT19300.029.7108214
TACGGGT953.7543643E-529.6850015
CTGTGCG157850.029.3278069
TAGTACT157600.029.256674
AGGTGTT19150.029.2054187
ACTGTGC162050.028.9445468
ACCTGGG18150.028.7449233
Produced by FastQC (version 0.11.5)