FastQCFastQC Report
Thu 26 May 2016
ERR580235_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580235_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5352340
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153830.28740700329201807No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113690.21241176756334612No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75160.14042456196728909No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81700.047.252181
GTATCAA142000.039.0083581
CAACGCA162800.032.5487065
ATCAACG168350.031.5903383
GTACATG191500.030.3494221
TATCAAC181550.030.1743952
AACGCAG180400.030.0130146
TACATGG197750.029.0816352
TCAACGC185700.028.7902374
GTACTAG31600.028.27271
ACATGGG196400.028.2278063
CATGGGG155750.026.869444
ACGCAGA197500.026.7716437
CGCAGAG203950.025.9713128
TAGTACT46350.025.3622024
GCATCGT32950.025.24718
CTAGTAC49550.025.1482073
CTAACGC16450.024.583143
ATGGGGG113850.024.4898345
CTGTGCG48600.023.8870479
Produced by FastQC (version 0.11.5)