FastQCFastQC Report
Thu 26 May 2016
ERR580235_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580235_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5352340
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135870.2538515864089352No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104640.19550327520299532No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76550.14302155692650317No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77700.047.133461
GTATCAA140300.041.349511
CAACGCA165500.033.737565
ATCAACG167850.033.209833
GTACTAG32250.031.487411
TATCAAC183350.031.4288942
AACGCAG184900.030.9086276
TCAACGC188800.029.6486554
GTACATG182750.027.4228571
ACGCAGA204550.027.3190387
TACATGG186150.026.66372
CGCAGAG211150.026.3093098
GTGGTAT27550.026.1085471
TAGGCAT43850.025.4023555
ACATGGG187600.025.1538353
TAGTACT46950.024.2256244
CTAGGCA49450.023.4760974
CTGTGCG47700.023.4499369
GCAGAGT233650.023.3734829
CATGGGG150550.023.2891314
Produced by FastQC (version 0.11.5)