FastQCFastQC Report
Thu 26 May 2016
ERR580234_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580234_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7180873
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA125450.041.7174071
GGTATCA69500.036.6696931
CAACGCA144050.034.4965635
ATCAACG148850.033.3885653
TATCAAC155400.033.1940732
AACGCAG169150.030.528816
TCAACGC165400.030.1885624
ACGCAGA187700.026.5355917
GTACATG254350.026.0108871
TACATGG259600.025.0870722
CGCAGAG202000.024.4709918
ACATGGG272850.023.3327713
CATGGGG224350.021.1036154
GCAGAGT230500.020.4505469
TATCACG5100.020.2841782
GTACTAG27200.019.8802451
GTATATA10650.019.8680731
AGAGTAC192350.017.9849410-11
ATGGGGG142650.017.8954325
TAGTACT37150.017.5917664
Produced by FastQC (version 0.11.5)