FastQCFastQC Report
Thu 26 May 2016
ERR580234_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580234_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7180873
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA127550.042.642191
GGTATCA71900.037.265771
CAACGCA152400.034.4456865
ATCAACG154300.033.9013523
TATCAAC163300.033.0969052
AACGCAG176650.030.7533686
TCAACGC173350.030.4734214
ACGCAGA200950.026.145827
GTACATG257350.025.4239461
TACATGG262800.024.5002732
CGCAGAG215700.024.183628
ACATGGG276050.022.5758233
CATGGGG224150.020.7575074
GCAGAGT240000.020.716679
GTACTAG27900.019.1057831
TAGTACT39950.018.8227024
ATGGGGG136800.018.7917795
GTATATA12750.018.1291121
GTGGTAT38950.017.9244121
AGAGTAC205450.017.71530510-11
Produced by FastQC (version 0.11.5)