FastQCFastQC Report
Thu 26 May 2016
ERR580233_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580233_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3796897
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135050.35568518187351406No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109060.28723454968623063No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66390.17485330784585412No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58200.052.9885641
GTATCAA106450.043.1469571
CAACGCA132950.033.7259335
ATCAACG133500.033.6565133
TATCAAC136200.033.370662
TCAACGC140000.032.3301664
AACGCAG143350.031.8256056
GTACATG134850.028.4821241
ACGCAGA159350.028.2176447
GTGGTAT22400.027.9128971
TACATGG137650.027.5216352
CGCAGAG166050.026.909668
ACATGGG138050.026.6233583
GTACTAG17050.025.9181961
TACCTGG24350.025.8655192
CATGGGG108050.025.838744
ATGGGGG77450.024.3954355
GCAGAGT182250.024.1344769
GCATCGT18250.022.3987068
TGGTATC27200.021.7729382
Produced by FastQC (version 0.11.5)