FastQCFastQC Report
Thu 26 May 2016
ERR580233_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580233_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3796897
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120540.31746976544267597No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104190.27440828655610094No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67590.17801378336046514No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64750.055.7509731
GTATCAA111850.040.4688951
ATCAACG142800.031.2028313
CAACGCA143100.031.169855
TATCAAC147150.030.6980882
TCAACGC150250.029.812084
AACGCAG155350.029.254646
GTGGTAT23350.027.1755221
ACGCAGA172200.026.1191087
CGCAGAG178350.025.1654138
GTACATG127050.025.0093861
TACATGG131100.024.0226152
ACATGGG131600.023.0365623
GCAGAGT196200.022.659429
GGGTTAG11200.022.242771
TACCTGG25000.021.9985492
TGGTATC27400.021.958752
GTACTAG13600.021.4281081
CATGGGG108200.021.2424
GAGTACT124500.020.68531612-13
Produced by FastQC (version 0.11.5)