##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR580233_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3796897
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.18219219536374	33.0	31.0	34.0	30.0	34.0
2	32.34402829468379	34.0	31.0	34.0	31.0	34.0
3	32.39231851693633	34.0	31.0	34.0	30.0	34.0
4	36.03083939332566	37.0	35.0	37.0	35.0	37.0
5	35.947480534762995	37.0	35.0	37.0	35.0	37.0
6	35.97977769741976	37.0	35.0	37.0	35.0	37.0
7	35.91399055597242	37.0	35.0	37.0	35.0	37.0
8	35.900629118988476	37.0	35.0	37.0	35.0	37.0
9	37.56627424973603	39.0	37.0	39.0	35.0	39.0
10-11	37.48939884331864	39.0	37.0	39.0	35.0	39.0
12-13	37.449935039059525	39.0	37.0	39.0	34.5	39.0
14-15	38.72605893707414	40.0	38.0	41.0	34.5	41.0
16-17	38.70012341656884	40.0	38.0	41.0	34.5	41.0
18-19	38.68535122232707	40.0	38.0	41.0	34.0	41.0
20-21	38.6434285681176	40.0	38.0	41.0	34.0	41.0
22-23	38.54983911862766	40.0	38.0	41.0	34.0	41.0
24-25	38.47043980913888	40.0	38.0	41.0	34.0	41.0
26-27	38.325480912439815	40.0	38.0	41.0	34.0	41.0
28-29	38.186059563901786	40.0	38.0	41.0	34.0	41.0
30-31	37.99324738069008	40.0	37.0	41.0	33.0	41.0
32-33	37.85725251435581	40.0	37.0	41.0	33.0	41.0
34-35	37.74583613935274	40.0	37.0	41.0	33.0	41.0
36-37	37.60242613902879	40.0	37.0	41.0	33.0	41.0
38-39	37.46796094284359	40.0	36.0	41.0	32.0	41.0
40-41	37.33601135348154	39.0	36.0	41.0	32.0	41.0
42-43	37.331386524311824	39.0	36.0	41.0	32.0	41.0
44-45	37.28865083777622	40.0	36.0	41.0	32.0	41.0
46-47	37.09295445728446	39.5	36.0	41.0	31.0	41.0
48-49	36.88659173530385	39.0	36.0	41.0	31.0	41.0
50-51	36.68929720769354	39.0	35.0	41.0	30.5	41.0
52-53	36.512931744000426	39.0	35.0	40.5	30.0	41.0
54-55	36.28390551547751	39.0	35.0	40.0	30.0	41.0
56-57	36.04785196964785	38.0	35.0	40.0	29.0	41.0
58-59	35.80742366727357	38.0	35.0	40.0	29.0	41.0
60-61	35.53661200185309	37.5	34.0	40.0	28.0	41.0
62-63	35.21569823990485	37.0	34.0	40.0	28.0	41.0
64-65	34.896125836439595	36.5	34.0	39.5	28.0	41.0
66-67	34.560209297223494	36.0	34.0	39.0	27.0	41.0
68-69	34.222072655644865	35.5	33.0	39.0	26.0	40.0
70-71	33.86832919091563	35.0	33.0	38.0	26.0	40.0
72-73	33.52905649007597	35.0	33.0	37.5	26.0	40.0
74-75	33.158129256600844	35.0	32.0	37.0	26.0	39.0
76-77	31.681359278379162	33.5	30.5	35.5	24.0	38.0
78-79	32.635895706415	35.0	32.0	36.0	26.0	38.5
80-81	32.6498427531745	35.0	32.0	36.0	26.0	37.5
82-83	32.415598711263435	35.0	32.0	35.0	25.5	37.0
84-85	32.185247848440454	35.0	32.0	35.0	25.0	36.5
86-87	31.962398900997314	34.0	32.0	35.0	25.0	36.0
88-89	31.76294273982149	34.0	31.5	35.0	24.5	36.0
90-91	31.580291090329816	34.0	31.0	35.0	24.0	35.5
92-93	31.396462032022463	34.0	31.0	35.0	24.0	35.0
94-95	31.229270375256426	34.0	31.0	35.0	23.0	35.0
96-97	31.06980752967489	34.0	31.0	35.0	23.0	35.0
98-99	30.927697801652243	34.0	31.0	35.0	21.0	35.0
100	28.883751389621576	32.0	26.0	34.0	17.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.08862268879627067
1101	2	-0.05387698957564879
1101	3	-0.04663101160738847
1101	4	-0.01877998909814238
1101	5	-0.08790403204224617
1101	6	-0.12409443278038879
1101	7	-0.09654477068618661
1101	8	-0.04724182254155096
1101	9	-0.08088273366755061
1101	10-11	-0.06390976510869706
1101	12-13	-0.10093246027689418
1101	14-15	-0.1411419821716393
1101	16-17	-0.13336497313957807
1101	18-19	-0.11022789271253686
1101	20-21	-0.1333758755046759
1101	22-23	-0.17594648683333247
1101	24-25	-0.20649502263639619
1101	26-27	-0.25414908991243124
1101	28-29	-0.1960784542580214
1101	30-31	-0.17313370881610268
1101	32-33	-0.12336869206988865
1101	34-35	-0.27818912907860494
1101	36-37	-0.2873414814607429
1101	38-39	-0.20914549263432747
1101	40-41	-0.33794593223787217
1101	42-43	-0.34193089707789426
1101	44-45	-0.22940897417760908
1101	46-47	-0.2667866731520405
1101	48-49	-0.21589090257025134
1101	50-51	-0.14899301561474942
1101	52-53	-0.1819516933399683
1101	54-55	-0.20850951739661383
1101	56-57	-0.20379094473575066
1101	58-59	-0.20473452421941118
1101	60-61	-0.22495422322756298
1101	62-63	-0.23746698017346546
1101	64-65	-0.2376101109060258
1101	66-67	-0.23794197150856178
1101	68-69	-0.19959427448659994
1101	70-71	-0.21262432413801235
1101	72-73	-0.1656634223937843
1101	74-75	-0.030248640783305802
1101	76-77	-0.10793688729876649
1101	78-79	-0.11367265509552027
1101	80-81	-0.07215379752521045
1101	82-83	-0.10261160244136391
1101	84-85	-0.09526042750617592
1101	86-87	-0.32735984292313347
1101	88-89	-0.25128310026126144
1101	90-91	-0.1545681608966909
1101	92-93	-0.14822882527722925
1101	94-95	-0.2075275519174049
1101	96-97	-0.25531703733418
1101	98-99	-0.368035801983126
1101	100	-0.32475197468264483
1102	1	-0.12217922996273245
1102	2	-0.10242187399773428
1102	3	-0.13069338781701845
1102	4	-0.0948609341410176
1102	5	-0.03038421684746595
1102	6	-0.06460008675408346
1102	7	0.0015627197766932
1102	8	-0.03733843422345018
1102	9	-0.09510724964820838
1102	10-11	-0.07121425058073072
1102	12-13	-0.07003279595428324
1102	14-15	-0.02733028870510168
1102	16-17	-0.007431274109443109
1102	18-19	-0.08305814039968595
1102	20-21	-0.13422265324634708
1102	22-23	-0.10758013373452258
1102	24-25	-0.15810249864548354
1102	26-27	-0.18175092322496056
1102	28-29	-0.25031249385759935
1102	30-31	-0.2955101471668726
1102	32-33	-0.24854058894415232
1102	34-35	-0.22802494354021263
1102	36-37	-0.325326203644579
1102	38-39	-0.40772489938084533
1102	40-41	-0.3131495003865439
1102	42-43	-0.2913506885430408
1102	44-45	-0.24717542346560606
1102	46-47	-0.20925446899335043
1102	48-49	-0.24214073460449015
1102	50-51	-0.23987241921638258
1102	52-53	-0.15879735512188375
1102	54-55	-0.1902143134822083
1102	56-57	-0.19162716612716935
1102	58-59	-0.3092969825456393
1102	60-61	-0.3243637461476254
1102	62-63	-0.34932141588316057
1102	64-65	-0.33387406794745544
1102	66-67	-0.2695883488643318
1102	68-69	-0.35499317847625633
1102	70-71	-0.2204134881148505
1102	72-73	-0.32524598419644946
1102	74-75	-0.2709139535585834
1102	76-77	-0.25123352070900395
1102	78-79	-0.2752727914376578
1102	80-81	-0.2953353041058193
1102	82-83	-0.2709169478345572
1102	84-85	-0.288579567534196
1102	86-87	-0.34120379534570233
1102	88-89	-0.31750143732557845
1102	90-91	-0.4416668736198055
1102	92-93	-0.32327726136113455
1102	94-95	-0.34250074728215196
1102	96-97	-0.42925860685072337
1102	98-99	-0.4801953556373455
1102	100	-0.26460784338249965
1103	1	-0.052943301571872325
1103	2	0.013795152024279389
1103	3	-0.015369293348825863
1103	4	-0.037096199778233085
1103	5	-0.09796722971758953
1103	6	-0.05314575921092057
1103	7	-0.027904021499367104
1103	8	-0.09404950935272893
1103	9	-0.1449467203977548
1103	10-11	-0.11293498921371281
1103	12-13	-0.04162616286184573
1103	14-15	-0.1020984241449554
1103	16-17	-0.04867578258038918
1103	18-19	-0.07995569932324997
1103	20-21	-0.13723580116739953
1103	22-23	-0.17932271942618172
1103	24-25	-0.17797667466808775
1103	26-27	-0.05113785626303269
1103	28-29	-0.06298835176639983
1103	30-31	-0.17996281981022832
1103	32-33	-0.208105671748676
1103	34-35	-0.17561382991566177
1103	36-37	-0.17068930930020798
1103	38-39	-0.1670808377725379
1103	40-41	-0.10802575991610297
1103	42-43	-0.048372106994584385
1103	44-45	0.028269653306665532
1103	46-47	0.01454543099832506
1103	48-49	-0.005607630655347862
1103	50-51	-0.023983396718477934
1103	52-53	-0.003684230615483841
1103	54-55	-0.04684274283544454
1103	56-57	0.05457092974589983
1103	58-59	0.03043023678613821
1103	60-61	-0.08121997226170663
1103	62-63	-0.04702291084900878
1103	64-65	0.05240266543111716
1103	66-67	-0.011661100958754389
1103	68-69	-0.07758514050484422
1103	70-71	-0.13453629199930361
1103	72-73	0.0020977627030944745
1103	74-75	-0.05813382108723175
1103	76-77	0.009044617548550349
1103	78-79	-0.018932481332448958
1103	80-81	-0.01507038123526172
1103	82-83	0.14089961227561076
1103	84-85	0.08583208978645729
1103	86-87	0.02311823148987102
1103	88-89	-0.011244765142578217
1103	90-91	-0.005873613070317418
1103	92-93	0.010342802215589586
1103	94-95	0.04196829577302097
1103	96-97	0.03304083080082876
1103	98-99	-0.053250550713123346
1103	100	0.1072683843983846
1104	1	-0.03724149317711323
1104	2	-0.007052628408061423
1104	3	-0.03424520797713626
1104	4	-0.03407108605357223
1104	5	-0.011140430680192992
1104	6	-0.004102270807379682
1104	7	-0.022595129707674744
1104	8	0.007756912383989345
1104	9	-0.03396311450629241
1104	10-11	-0.031047719818431574
1104	12-13	-0.050503693630901125
1104	14-15	-0.07708407618086
1104	16-17	-0.07401901663760668
1104	18-19	-0.09867623812194637
1104	20-21	-0.09600839884816992
1104	22-23	-0.1491423064717523
1104	24-25	-0.021818380500299384
1104	26-27	-0.19280913149382428
1104	28-29	-0.05833041361380964
1104	30-31	-0.1128498913225684
1104	32-33	-0.09410290022687207
1104	34-35	-0.04236128427637453
1104	36-37	-0.15122656439105242
1104	38-39	-0.06909367288962898
1104	40-41	-0.16441304120757394
1104	42-43	-0.04875902327616899
1104	44-45	-0.06387118770164335
1104	46-47	-0.07695983437082532
1104	48-49	0.02878461337783733
1104	50-51	-0.0543938268324311
1104	52-53	-0.07509501993630607
1104	54-55	-0.0455689076609076
1104	56-57	-0.1795594095392019
1104	58-59	-0.18441920969291203
1104	60-61	-0.17329857589288622
1104	62-63	-0.09919139743910677
1104	64-65	-0.2479705122033522
1104	66-67	-0.18082272721996873
1104	68-69	-0.1732129430002587
1104	70-71	-0.09592271444397937
1104	72-73	-0.131031178634764
1104	74-75	-0.12265417836749748
1104	76-77	-0.07649978686151471
1104	78-79	-0.006418343727787601
1104	80-81	-0.05454186258928928
1104	82-83	-0.1127119690412215
1104	84-85	-0.057009436732471386
1104	86-87	0.0018884673419634623
1104	88-89	-0.08956680483406743
1104	90-91	-0.08410207908290346
1104	92-93	9.614968729252382E-4
1104	94-95	0.0038554462064190886
1104	96-97	-0.012752821973215589
1104	98-99	-0.09877806253532384
1104	100	-0.03712929416195365
1105	1	-0.022865210648710388
1105	2	-0.06097050485405475
1105	3	-0.025302880888048662
1105	4	0.01851748231432282
1105	5	-0.0693006849947082
1105	6	-0.03528376205314743
1105	7	-0.02319098805393338
1105	8	0.019623578123173502
1105	9	-0.04706657237675671
1105	10-11	-0.06713909496711779
1105	12-13	-0.04490689621299282
1105	14-15	-0.07759771358790601
1105	16-17	-0.12479698532131067
1105	18-19	-0.16308862273786673
1105	20-21	-0.14105761315823173
1105	22-23	-0.17804505343020338
1105	24-25	-0.17012867759473949
1105	26-27	-0.19396460697710438
1105	28-29	-0.18294473341291706
1105	30-31	-0.20464588322568034
1105	32-33	-0.11959953000309298
1105	34-35	-0.1531569909799586
1105	36-37	-0.1494062261861373
1105	38-39	-0.2195379581169803
1105	40-41	-0.10242227889008859
1105	42-43	-0.11471885883428712
1105	44-45	-0.16032970508143052
1105	46-47	-0.0891499581196058
1105	48-49	-0.09888245442313348
1105	50-51	-0.1831132809187892
1105	52-53	-0.2859066560773371
1105	54-55	-0.1993503056951198
1105	56-57	-0.21116403951019436
1105	58-59	-0.2891069162017246
1105	60-61	-0.24675366392215636
1105	62-63	-0.22769048271376846
1105	64-65	-0.19995209248701684
1105	66-67	-0.1924936486927109
1105	68-69	-0.29939544287852016
1105	70-71	-0.22545818700570663
1105	72-73	-0.2626168184903577
1105	74-75	-0.21702809582657068
1105	76-77	-0.11444210699451318
1105	78-79	-0.15775133853158252
1105	80-81	-0.14617779494830074
1105	82-83	-0.1228278574377768
1105	84-85	-0.10605721808876467
1105	86-87	-0.10370283476781594
1105	88-89	-0.11691458827719003
1105	90-91	-0.1512762386137858
1105	92-93	-0.096297318227343
1105	94-95	-0.0819433559555307
1105	96-97	-0.05745374504585854
1105	98-99	-8.631493820629998E-4
1105	100	-0.10855466579086226
1106	1	0.03015824072006268
1106	2	-0.09121342739913985
1106	3	-0.010674272086774295
1106	4	0.020048828665814256
1106	5	0.003671378912613932
1106	6	0.027506109945356627
1106	7	-0.06748043960957517
1106	8	-0.038605890250181574
1106	9	0.0033706882043844644
1106	10-11	-0.022472179149119142
1106	12-13	0.02639040360948286
1106	14-15	0.0026629609893120687
1106	16-17	-0.016352623927303966
1106	18-19	0.0015026133641669048
1106	20-21	0.08109131704825501
1106	22-23	0.09447293218130426
1106	24-25	-0.06638626892426913
1106	26-27	-0.023230233436969172
1106	28-29	7.4768927230906E-4
1106	30-31	-0.04980611867368623
1106	32-33	-0.06732072966673996
1106	34-35	-0.02658406750055775
1106	36-37	-0.14432400624780684
1106	38-39	-0.13780947077699324
1106	40-41	-0.10031165570032385
1106	42-43	-0.1356998921894217
1106	44-45	-0.08341108413403475
1106	46-47	-0.12250696082912782
1106	48-49	-0.1346383172653276
1106	50-51	-0.06167676779162434
1106	52-53	-0.03212827618385461
1106	54-55	-0.08638478201017818
1106	56-57	-0.047901402866266096
1106	58-59	-0.03353838401448428
1106	60-61	-0.05902732551236767
1106	62-63	0.02225579403106792
1106	64-65	-0.00390752554317686
1106	66-67	-0.018175700453902266
1106	68-69	-0.034174009635506764
1106	70-71	0.01233775805642523
1106	72-73	0.02014642545993439
1106	74-75	-0.057661563078745814
1106	76-77	-0.15188721584249976
1106	78-79	-0.13589671833164374
1106	80-81	0.015852009779337095
1106	82-83	-0.01463389312871044
1106	84-85	0.018862897766595665
1106	86-87	-0.09032808002615766
1106	88-89	-0.1271962849977335
1106	90-91	-0.011887282246412667
1106	92-93	-0.01653136475635364
1106	94-95	0.01643560321479498
1106	96-97	-0.042986306010075026
1106	98-99	-0.08184978864208148
1106	100	0.04249457274039159
1107	1	-0.0250454186520912
1107	2	-0.04216164095372932
1107	3	-0.05829279747552363
1107	4	0.008563939921756969
1107	5	-0.013596165936526461
1107	6	0.007874167415756972
1107	7	-0.00503266263951474
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2316	64-65	-0.20039112248357327
2316	66-67	-0.2481539176273131
2316	68-69	-0.22218822531731774
2316	70-71	-0.22185400357850682
2316	72-73	-0.1457934648528436
2316	74-75	-0.11680398365556499
2316	76-77	-0.15504663520966133
2316	78-79	-0.1271203765731883
2316	80-81	-0.189211928955892
2316	82-83	-0.23345307293025286
2316	84-85	-0.2297682857821357
2316	86-87	-0.22136747101652432
2316	88-89	-0.24663416074012545
2316	90-91	-0.24969481119164882
2316	92-93	-0.3049894225831693
2316	94-95	-0.26494530975394426
2316	96-97	-0.33109010868222555
2316	98-99	-0.23784165759951037
2316	100	-0.4231667793410274
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	14.0
13	63.0
14	209.0
15	744.0
16	2126.0
17	4291.0
18	7574.0
19	11611.0
20	16399.0
21	22909.0
22	28756.0
23	30267.0
24	29757.0
25	31401.0
26	35611.0
27	43282.0
28	53233.0
29	65414.0
30	80912.0
31	101498.0
32	127986.0
33	169105.0
34	244627.0
35	355458.0
36	584032.0
37	905493.0
38	739415.0
39	104694.0
40	15.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.19294626574137	20.446157752396566	13.77641647290109	24.584479508960978
2	15.338917459914361	21.70960047924156	38.65115313548637	24.300328925357707
3	17.765587542923786	26.905937191026446	30.42867685442075	24.89979841162902
4	12.772851754168332	17.729734342382432	37.14086601444042	32.356547889008816
5	13.16912771405739	37.82891739024849	34.487183606901084	14.514771288793039
6	30.812492839543893	37.17974818898073	17.841789146131244	14.16596982534413
7	26.776097326635877	32.730353259953475	22.68603774061963	17.80751167279102
8	26.15150362631852	35.050391288407155	20.236453456275573	18.56165162899875
9	26.640228843354446	15.3581682417952	20.445039326196333	37.556563588654015
10-11	25.16382822408401	25.884497266643464	28.273282188823824	20.678392320448705
12-13	25.581949351232876	23.74444743519308	28.313682183384632	22.359921030189412
14-15	23.099992704511376	25.068391913648806	26.103745535463847	25.727869846375974
16-17	22.388937481251116	27.712276853732774	26.867349172073062	23.031436492943048
18-19	22.115767269735326	26.931379332232247	29.07567808022368	21.877175317808746
20-21	23.064214290201043	26.30204969147764	28.714683088534205	21.919052929787114
22-23	22.984860701944157	26.00917789409379	28.556611740735487	22.44934966322657
24-25	22.56747004769718	26.08925246321312	28.77974489513731	22.563532593952388
26-27	22.472327818672397	26.3224065095553	28.52713396755731	22.67813170421499
28-29	22.33378137551804	26.78800634045315	28.573994581617402	22.304217702411407
30-31	23.06267333538768	26.209508985127066	28.59809367287974	22.12972400660551
32-33	22.95475088810508	26.435348478527455	28.28373693126509	22.326163702102374
34-35	22.981799065571323	26.53943101223891	28.45252221865642	22.02624770353335
36-37	22.928086320699244	26.406899840401948	28.333151817269908	22.3318620216289
38-39	22.920398919316973	26.45960159689933	28.38475918772838	22.235240296055316
40-41	22.875858050115063	26.084354762774865	28.569906598055578	22.46988058905449
42-43	22.679640955135195	26.391483219814894	28.130264384708987	22.798611440340924
44-45	22.493313082837705	26.737146573826582	28.253800358962117	22.5157399843736
46-47	22.573005876921897	26.376813846289792	27.834409524545734	23.21577075224258
48-49	22.84056423458034	26.511945147972177	27.835531364532695	22.81195925291479
50-51	22.737497700264036	26.641313490656128	27.669553919046397	22.951634890033443
52-53	22.616602047063687	26.84950759296248	27.467490573738058	23.06639978623578
54-55	22.49316579534234	26.67532503790781	27.6490757395019	23.182433427247954
56-57	22.802348523178523	26.67100632142751	27.33321410915439	23.193431046239578
58-59	22.496362263626153	26.770592208600164	27.711932631321655	23.021112896452024
60-61	22.789884150907515	26.852596430942928	27.504486278034136	22.853033140115425
62-63	22.799884269761023	26.71142938829036	27.36448611915342	23.124200222795196
64-65	22.42837929078638	26.52819664795288	27.442187794865326	23.601236266395418
66-67	22.536349013744182	26.43338364852098	27.39528958185893	23.63497775587591
68-69	22.21644836709568	26.54932425483139	27.52817267449754	23.706054703575386
70-71	22.285939890180742	26.28558801343453	27.53403933434191	23.894432762042825
72-73	22.31209375413193	26.572726932714964	27.34916060299411	23.766018710159
74-75	22.465533617201505	26.603712060112766	27.178292250061766	23.752462072623963
76-77	22.252308001885304	26.50327458858417	27.169438806195796	24.07497860333473
78-79	22.081144039645054	26.43526777806998	27.40009558070281	24.08349260158215
80-81	22.033162180207253	26.663330519556627	27.197096498494123	24.106410801741994
82-83	22.344534645631548	26.317473762727506	27.48705585748458	23.850935734156366
84-85	22.411407702743595	26.53255910301377	26.98561131562528	24.070421878617353
86-87	21.90705948322658	26.74262810492445	27.173694034715474	24.1766183771335
88-89	21.90347575766355	26.2006205111052	27.317790706555662	24.578113024675588
90-91	22.149105681699513	26.115756286723883	27.28290083004759	24.452237201529012
92-93	22.080695537846907	26.19489271348201	27.171955777479933	24.55245597119115
94-95	21.655329514863666	26.11461361369508	27.325182804823733	24.90487406661752
96-97	21.625516419275296	26.04508564803475	27.29426324885733	25.03513468383262
98-99	21.47818790267059	26.151537147489567	27.150144409637704	25.220130540202135
100	21.192811180501124	26.16039522810211	27.162740301551587	25.48405328984518
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1012.0
1	728.0
2	777.0
3	1064.0
4	804.5
5	666.5
6	1019.5
7	1842.0
8	2804.0
9	3378.0
10	3074.0
11	2637.0
12	2790.5
13	3031.5
14	3386.5
15	3854.0
16	4192.5
17	4552.5
18	5120.5
19	5688.0
20	6192.5
21	6844.0
22	7761.0
23	9079.5
24	10698.0
25	12728.0
26	15457.0
27	18563.5
28	22750.0
29	28410.5
30	34638.0
31	41569.5
32	50310.5
33	58532.5
34	65872.5
35	73289.0
36	81485.5
37	93112.5
38	101025.0
39	103013.5
40	104569.5
41	106006.0
42	109078.0
43	115187.0
44	123388.5
45	133972.5
46	143457.0
47	151519.0
48	158216.0
49	161499.0
50	162067.5
51	162761.0
52	160723.0
53	155160.5
54	149066.5
55	137233.5
56	122952.5
57	107318.0
58	91782.0
59	79529.5
60	65376.0
61	51466.0
62	40757.5
63	31298.0
64	22632.5
65	15664.0
66	11030.0
67	8084.5
68	6028.0
69	4430.0
70	3318.5
71	2461.0
72	1808.0
73	1284.5
74	911.0
75	642.0
76	419.0
77	231.0
78	139.0
79	83.5
80	42.5
81	24.0
82	14.0
83	8.0
84	6.5
85	2.0
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005609844038434543
2	0.023677229063627483
3	0.0013432020937096793
4	0.0014485512775300462
5	0.007242756387650231
6	5.2674591910183496E-5
7	8.691307665180277E-4
8	0.005504494854614176
9	0.0016855869411258719
10-11	2.2386701561827985E-4
12-13	7.901188786527526E-5
14-15	7.901188786527526E-4
16-17	1.0534918382036699E-4
18-19	0.0010008172462934865
20-21	2.2386701561827985E-4
22-23	0.0014880572214626838
24-25	7.111069907874772E-4
26-27	0.0020674777324747023
28-29	1.9752971966318814E-4
30-31	0.0017645988289911475
32-33	0.0012905275017994957
34-35	8.296248225853902E-4
36-37	0.004516846256298235
38-39	0.0010271545422485782
40-41	0.0035423663059598407
42-43	0.0041349554649494046
44-45	0.0030946322747232805
46-47	0.0025415490596663536
48-49	0.009362908712035116
50-51	0.006992552076076859
52-53	0.005017254879444978
54-55	0.004898737047647065
56-57	0.0033711738822517438
58-59	0.006228770493379198
60-61	0.00842793470562936
62-63	0.003595040897870024
64-65	0.003753064673600574
66-67	0.004319316536635046
68-69	0.00233085069202562
70-71	0.0029761144429253676
72-73	0.0063999629170872945
74-75	0.007874851490572432
76-77	0.004227136000792226
78-79	0.0027785847232621795
80-81	0.0026864041874193586
82-83	0.005241121895063258
84-85	0.0024757058197786242
86-87	0.0038452452094433955
88-89	7.111069907874772E-4
90-91	0.0017514301810136015
92-93	2.1069836764073399E-4
94-95	0.002317682044048074
96-97	0.002080646380452248
98-99	0.0015539004613504134
100	2.6337295955091748E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3796897.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.88793026199375
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.97450157333904	49.816295187312065
2	13.431091241480933	17.16169241156418
3	3.948221952198238	7.567311862227415
4	1.6323031261375263	4.171378731564348
5	0.8577816042927008	2.7400945657536595
6	0.5056308497162934	1.938222507899232
7	0.34895652592145987	1.560587713477649
8	0.2415977537897114	1.2348144356457136
9	0.17777739964286862	1.0222047099487965
>10	0.8426296455539061	9.034231284769145
>50	0.028721163419921206	1.242203316448026
>100	0.009826405736463843	1.104819170869597
>500	6.244932014771963E-4	0.29060782069915125
>1k	1.9215175395675963E-4	0.21839320406729937
>5k	4.8037938489189906E-5	0.2074367153303649
>10k+	9.607587697837981E-5	0.6897063624233304
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12054	0.31746976544267597	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10419	0.27440828655610094	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6759	0.17801378336046514	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	2.633729595509175E-5
3	0.0	0.0	0.0	0.0	2.633729595509175E-5
4	0.0	0.0	0.0	0.0	2.633729595509175E-5
5	0.0	0.0	0.0	0.0	2.633729595509175E-5
6	5.26745919101835E-5	0.0	0.0	0.0	2.633729595509175E-5
7	5.26745919101835E-5	0.0	0.0	0.0	2.633729595509175E-5
8	5.26745919101835E-5	0.0	0.0	0.0	2.633729595509175E-5
9	5.26745919101835E-5	0.0	0.0	0.0	2.633729595509175E-5
10-11	5.26745919101835E-5	0.0	0.0	0.0	2.633729595509175E-5
12-13	5.26745919101835E-5	1.3168647977545876E-5	0.0	0.0	3.950594393263763E-5
14-15	5.26745919101835E-5	5.2674591910183496E-5	0.0	0.0	1.4485512775300462E-4
16-17	7.901188786527524E-5	7.901188786527524E-5	0.0	1.3168647977545876E-5	2.2386701561827988E-4
18-19	7.901188786527524E-5	7.901188786527524E-5	0.0	3.950594393263763E-5	2.3703566359582575E-4
20-21	7.901188786527524E-5	7.901188786527524E-5	0.0	7.901188786527526E-5	2.3703566359582575E-4
22-23	7.901188786527524E-5	7.901188786527524E-5	0.0	3.2921619943864687E-4	2.633729595509175E-4
24-25	7.901188786527524E-5	7.901188786527524E-5	0.0	6.716010468548396E-4	3.16047551461101E-4
26-27	7.901188786527524E-5	7.901188786527524E-5	0.0	0.0015407318133728674	3.16047551461101E-4
28-29	7.901188786527524E-5	7.901188786527524E-5	0.0	0.004859231103714428	3.16047551461101E-4
30-31	7.901188786527524E-5	7.901188786527524E-5	0.0	0.016078919180583514	3.16047551461101E-4
32-33	7.901188786527524E-5	7.901188786527524E-5	0.0	0.04499727013927425	3.16047551461101E-4
34-35	7.901188786527524E-5	7.901188786527524E-5	0.0	0.09102169482079708	3.16047551461101E-4
36-37	7.901188786527524E-5	7.901188786527524E-5	0.0	0.1666624088038206	3.16047551461101E-4
38-39	7.901188786527524E-5	7.901188786527524E-5	0.0	0.27486918923531506	3.16047551461101E-4
40-41	7.901188786527524E-5	7.901188786527524E-5	0.0	0.39758781973806506	3.2921619943864687E-4
42-43	7.901188786527524E-5	1.05349183820367E-4	0.0	0.5230982036120548	3.4238484741619277E-4
44-45	7.901188786527524E-5	1.05349183820367E-4	0.0	0.6782907200274328	3.4238484741619277E-4
46-47	7.901188786527524E-5	1.05349183820367E-4	0.0	0.8637579581431891	3.555534953937386E-4
48-49	7.901188786527524E-5	1.05349183820367E-4	0.0	1.0624070128844685	4.21396735281468E-4
50-51	7.901188786527524E-5	1.05349183820367E-4	0.0	1.2725522973101455	4.21396735281468E-4
52-53	7.901188786527524E-5	1.05349183820367E-4	0.0	1.482631738495935	5.135772711242891E-4
54-55	7.901188786527524E-5	1.05349183820367E-4	0.0	1.726185882840646	6.189264549446561E-4
56-57	7.901188786527524E-5	1.05349183820367E-4	0.0	2.004176041646639	6.32095102922202E-4
58-59	7.901188786527524E-5	1.05349183820367E-4	0.0	2.3203816168834708	6.32095102922202E-4
60-61	7.901188786527524E-5	1.05349183820367E-4	0.0	2.647148447798294	6.32095102922202E-4
62-63	7.901188786527524E-5	1.05349183820367E-4	0.0	2.9877423590895407	6.584323988772937E-4
64-65	7.901188786527524E-5	1.05349183820367E-4	0.0	3.3316284323751737	6.584323988772937E-4
66-67	7.901188786527524E-5	1.05349183820367E-4	0.0	3.7219339897816557	6.716010468548396E-4
68-69	7.901188786527524E-5	1.05349183820367E-4	0.0	4.148743039381896	6.847696948323855E-4
70-71	7.901188786527524E-5	1.05349183820367E-4	0.0	4.6019420595291365	7.111069907874772E-4
72-73	7.901188786527524E-5	1.05349183820367E-4	0.0	5.053995407302331	8.42793470562936E-4
74-75	7.901188786527524E-5	1.05349183820367E-4	0.0	5.508629810079125	8.42793470562936E-4
76-77	7.901188786527524E-5	1.05349183820367E-4	0.0	6.008696048378452	8.42793470562936E-4
78-79	7.901188786527524E-5	1.05349183820367E-4	0.0	6.5593957381514425	8.559621185404819E-4
80-81	7.901188786527524E-5	1.05349183820367E-4	0.0	7.135405569337277	8.954680624731195E-4
82-83	7.901188786527524E-5	1.05349183820367E-4	0.0	7.729627640676057	9.48142654383303E-4
84-85	7.901188786527524E-5	1.05349183820367E-4	0.0	8.323415146631579	9.48142654383303E-4
86-87	7.901188786527524E-5	1.05349183820367E-4	0.0	8.931991044265883	9.48142654383303E-4
88	7.901188786527524E-5	1.05349183820367E-4	0.0	9.407813801638548	9.744799503383948E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6475	0.0	55.750973	1
GTATCAA	11185	0.0	40.468895	1
ATCAACG	14280	0.0	31.202831	3
CAACGCA	14310	0.0	31.16985	5
TATCAAC	14715	0.0	30.698088	2
TCAACGC	15025	0.0	29.81208	4
AACGCAG	15535	0.0	29.25464	6
GTGGTAT	2335	0.0	27.175522	1
ACGCAGA	17220	0.0	26.119108	7
CGCAGAG	17835	0.0	25.165413	8
GTACATG	12705	0.0	25.009386	1
TACATGG	13110	0.0	24.022615	2
ACATGGG	13160	0.0	23.036562	3
GCAGAGT	19620	0.0	22.65942	9
GGGTTAG	1120	0.0	22.24277	1
TACCTGG	2500	0.0	21.998549	2
TGGTATC	2740	0.0	21.95875	2
GTACTAG	1360	0.0	21.428108	1
CATGGGG	10820	0.0	21.242	4
GAGTACT	12450	0.0	20.685316	12-13
>>END_MODULE