FastQCFastQC Report
Thu 26 May 2016
ERR580232_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580232_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6993747
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185000.26452200801658965No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150300.21490625840482935No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91470.1307882598555538No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102200.046.298531
GTATCAA178450.039.6416171
CAACGCA202800.033.4860085
ATCAACG207550.032.7250333
TATCAAC223750.031.4275632
AACGCAG231300.030.1572956
TCAACGC235900.028.989524
GTACATG260200.028.4161571
GTACTAG51100.027.9816171
GTGGTAT34100.027.8623071
TACATGG260750.027.7076322
ACGCAGA249150.027.222647
ACATGGG258050.026.904083
CTGTGCG70700.026.7915619
TAGTACT70400.026.65664
CGCAGAG257100.026.3980148
GGGCACC65950.025.36849694
CTAGTAC79000.024.9472453
CATGGGG216650.024.7703134
GCATCGT55100.023.7990048
Produced by FastQC (version 0.11.5)