FastQCFastQC Report
Thu 26 May 2016
ERR580232_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580232_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6993747
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157650.22541564629089383No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132360.18925477287068004No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85260.12190889947834829No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104100.047.0086331
GTATCAA180150.039.062961
CAACGCA206500.032.45685
ATCAACG210050.031.9087093
GTACTAG48650.031.6933841
TATCAAC231750.030.1382242
AACGCAG235250.029.3478726
TCAACGC242750.027.9004334
GTACATG251700.027.6598261
TACATGG254000.027.1465722
TAGGCAT62600.026.5037045
ACGCAGA253150.026.492967
ACATGGG252500.025.9485913
CGCAGAG263750.025.4462228
CTAGGCA70350.025.1205964
CATGGGG212700.024.5721684
GGGCACC63750.024.25927294
TAGTACT69700.024.2084984
CTGTGCG71550.024.1072189
GTGGTAT34450.023.8795661
Produced by FastQC (version 0.11.5)