FastQCFastQC Report
Thu 26 May 2016
ERR580231_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580231_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4956786
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83550.16855680273467527No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65330.13179911337709557No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62350.046.9059561
GTATCAA112000.041.519521
CAACGCA130950.034.3932085
ATCAACG133950.033.661423
TATCAAC141350.032.7653242
AACGCAG145350.031.586666
TCAACGC147850.030.6869534
GTACTAG23800.027.6582781
ACGCAGA165200.027.0790587
CGCAGAG170200.025.9241168
GTACATG192200.024.2434441
GCATCGT23150.023.5453368
TACATGG197400.023.104612
GTGGTAT29450.022.990651
GCAGAGT191800.022.520319
ACATGGG199700.021.9429173
GTATAAG29550.020.685211
CATGGGG159300.020.541934
TAGGCAT34800.019.8586965
TAGTACT35000.019.7468134
Produced by FastQC (version 0.11.5)