FastQCFastQC Report
Thu 26 May 2016
ERR580231_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580231_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4956786
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73370.14801930121655443No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60530.1221154191445828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70200.047.505021
GTATCAA118700.040.0162121
CAACGCA140600.032.6243255
ATCAACG143350.031.9325773
TATCAAC151600.031.2789042
AACGCAG157700.029.591896
TCAACGC157750.029.0477374
GTACTAG28550.026.5997161
ACGCAGA177700.025.7324187
CGCAGAG183800.024.7763798
GCAGAGT205900.021.8431439
GCATCGT30400.021.797428
GTACATG200850.021.793821
TACATGG201750.021.127752
GTGGTAT29600.020.8754961
CGTTAAC7700.020.2153111
ACATGGG205550.019.7774963
CTAGTAC36750.019.1825623
GAGTACT85500.019.12736512-13
TAGTACT36350.018.8766774
Produced by FastQC (version 0.11.5)