##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR580231_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4956786
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.45881081006927	34.0	31.0	34.0	31.0	34.0
2	32.67646555651182	34.0	31.0	34.0	31.0	34.0
3	32.756439959280065	34.0	31.0	34.0	31.0	34.0
4	36.24492100324686	37.0	35.0	37.0	35.0	37.0
5	36.16850919123803	37.0	35.0	37.0	35.0	37.0
6	36.19695504304604	37.0	37.0	37.0	35.0	37.0
7	36.16610037229769	37.0	37.0	37.0	35.0	37.0
8	36.1546639294091	37.0	37.0	37.0	35.0	37.0
9	37.93049609162066	39.0	38.0	39.0	35.0	39.0
10-11	37.87631541890249	39.0	38.0	39.0	35.0	39.0
12-13	37.83704077601897	39.0	38.0	39.0	35.0	39.0
14-15	39.21664602829333	41.0	39.0	41.0	36.0	41.0
16-17	39.20045761507558	41.0	39.0	41.0	36.0	41.0
18-19	39.19343078357629	41.0	39.0	41.0	36.0	41.0
20-21	39.17619189127794	40.5	39.0	41.0	36.0	41.0
22-23	39.10481590288546	40.0	39.0	41.0	35.0	41.0
24-25	39.018414654173085	40.0	38.5	41.0	35.0	41.0
26-27	38.876753606066515	40.0	38.0	41.0	35.0	41.0
28-29	38.770480609814506	40.0	38.0	41.0	35.0	41.0
30-31	38.66354518835391	40.0	38.0	41.0	35.0	41.0
32-33	38.52851938736109	40.0	38.0	41.0	34.0	41.0
34-35	38.50164370622416	40.0	38.0	41.0	34.5	41.0
36-37	38.55810942816575	40.0	38.0	41.0	34.5	41.0
38-39	38.46841733736336	40.0	38.0	41.0	34.0	41.0
40-41	38.34735572606927	40.0	38.0	41.0	34.0	41.0
42-43	38.32999598933664	40.0	38.0	41.0	34.0	41.0
44-45	38.24906905402008	40.0	38.0	41.0	34.0	41.0
46-47	38.06761538626037	40.0	37.5	41.0	33.0	41.0
48-49	37.87153903759412	40.0	37.0	41.0	33.0	41.0
50-51	37.704648838985584	40.0	37.0	41.0	33.0	41.0
52-53	37.52617845515219	40.0	36.0	41.0	33.0	41.0
54-55	37.328196436158436	39.5	36.0	41.0	32.0	41.0
56-57	37.112322077249246	39.0	35.5	41.0	32.0	41.0
58-59	36.87814805803599	39.0	35.0	41.0	31.5	41.0
60-61	36.61289180125993	38.5	35.0	40.0	31.0	41.0
62-63	36.31965410651176	38.0	35.0	40.0	31.0	41.0
64-65	36.0205955028117	37.0	35.0	40.0	31.0	41.0
66-67	35.691794239250996	37.0	35.0	39.5	30.5	41.0
68-69	35.33532807347342	36.0	34.5	39.0	30.0	41.0
70-71	35.00300436613564	36.0	34.0	39.0	30.0	40.0
72-73	34.64494129865602	35.0	34.0	38.0	29.0	40.0
74-75	34.28848602703445	35.0	34.0	37.0	29.0	39.0
76-77	33.06590782817737	34.5	32.0	36.0	27.0	39.0
78-79	33.712749551826526	35.0	33.5	36.0	29.0	39.0
80-81	33.65799804550771	35.0	34.0	36.0	29.0	37.5
82-83	33.454779871473164	35.0	34.0	35.5	29.0	37.0
84-85	33.24205261231774	35.0	33.5	35.0	29.0	37.0
86-87	33.07384896180711	35.0	33.0	35.0	29.0	36.0
88-89	32.91778220806789	35.0	33.0	35.0	29.0	36.0
90-91	32.76130288860564	35.0	33.0	35.0	29.0	36.0
92-93	32.64857782845578	35.0	33.0	35.0	27.5	35.0
94-95	32.55414365276209	35.0	33.0	35.0	27.0	35.0
96-97	32.45597953996804	35.0	33.0	35.0	27.0	35.0
98-99	32.382160839705406	35.0	33.0	35.0	27.0	35.0
100	31.083350178926427	34.0	30.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.18546089634808283
1101	2	0.10156399584983689
1101	3	0.08660685813705982
1101	4	0.07722075507712844
1101	5	0.0256067106799307
1101	6	0.002602480724632983
1101	7	-0.007988597191669555
1101	8	0.022594950498699973
1101	9	0.025961782332821315
1101	10-11	0.004974115729510231
1101	12-13	-0.024032579785838948
1101	14-15	0.0010106617813434582
1101	16-17	0.054243727437985
1101	18-19	0.02807461169114589
1101	20-21	0.04798034633966353
1101	22-23	0.06893284577054004
1101	24-25	-0.0032730693519127385
1101	26-27	0.012961359659357186
1101	28-29	-4.1214637811037846E-5
1101	30-31	-0.027583633113266615
1101	32-33	0.06530207480047068
1101	34-35	0.016306879095388638
1101	36-37	0.019797760282024512
1101	38-39	0.01728189279874215
1101	40-41	0.06467759020579678
1101	42-43	-0.00798776044307914
1101	44-45	-0.05792803786626877
1101	46-47	0.06558010187813323
1101	48-49	-0.038447084957418554
1101	50-51	-0.0652072896846434
1101	52-53	-0.003974251582654631
1101	54-55	-0.0720129438470849
1101	56-57	-0.09362049042529463
1101	58-59	-0.1533865923655
1101	60-61	-0.19746503486158673
1101	62-63	-0.1745268217439815
1101	64-65	-0.15803860136939107
1101	66-67	-0.20256290463245819
1101	68-69	-0.2035496240511847
1101	70-71	-0.19869246103009885
1101	72-73	-0.16576681588771436
1101	74-75	-0.1789269701893872
1101	76-77	-0.10295691699699461
1101	78-79	-0.1761658625245346
1101	80-81	-0.15556424789207313
1101	82-83	-0.1072094042740801
1101	84-85	-0.11873968152353598
1101	86-87	-0.0722769085138566
1101	88-89	-0.11167931981228207
1101	90-91	-0.09257766158489744
1101	92-93	-0.13837184898485333
1101	94-95	-0.1470774030327604
1101	96-97	-0.21560970972092974
1101	98-99	-0.25409541940575764
1101	100	-0.2057026951215022
1102	1	0.05508717812187314
1102	2	0.03772504350763484
1102	3	-0.0014821774573974267
1102	4	0.0254308069748177
1102	5	-0.0369093253718944
1102	6	0.007996318656431356
1102	7	-0.0011718415679666805
1102	8	-0.009617502538048939
1102	9	-0.02657416911853261
1102	10-11	-0.05694621517129406
1102	12-13	-0.011349476647211532
1102	14-15	0.03871407215287803
1102	16-17	-0.09922397809045691
1102	18-19	-0.06230059885759687
1102	20-21	-0.08471020261986695
1102	22-23	-0.09105384176496045
1102	24-25	-0.06167260475795189
1102	26-27	-0.14662174938145256
1102	28-29	-0.1190091472716901
1102	30-31	-0.12651024657350263
1102	32-33	-0.14626664240815757
1102	34-35	-0.1262629239950286
1102	36-37	-0.10457301159416943
1102	38-39	-0.18130936262549113
1102	40-41	-0.15884915089143448
1102	42-43	-0.04346030654468791
1102	44-45	-0.08096345743097544
1102	46-47	-0.1064154985339556
1102	48-49	-0.14392219130020578
1102	50-51	-0.13405669519348606
1102	52-53	-0.10148183607505956
1102	54-55	-0.1029960468475366
1102	56-57	-0.13485231582198054
1102	58-59	-0.09756097775463246
1102	60-61	-0.11582366168008207
1102	62-63	-0.08693352937845589
1102	64-65	-0.1417959141939491
1102	66-67	-0.09924734296549076
1102	68-69	-0.07789403353658741
1102	70-71	-0.10096152165056793
1102	72-73	-0.12098255223143184
1102	74-75	-0.19388067364634765
1102	76-77	-0.1011460151860959
1102	78-79	-0.03707007180185684
1102	80-81	-0.0684745749021829
1102	82-83	-0.051773313480069305
1102	84-85	-0.028052030749115886
1102	86-87	-0.06354802581533647
1102	88-89	-0.034535996248131084
1102	90-91	-0.11280102041997253
1102	92-93	-0.11767621744323975
1102	94-95	-0.05742393572202786
1102	96-97	-0.15472683907667317
1102	98-99	-0.12190931888994072
1102	100	-0.016148400144214037
1103	1	0.11125871186186487
1103	2	0.07327105757374852
1103	3	0.04666136324780723
1103	4	0.0047894236999113105
1103	5	-0.01675493131965311
1103	6	-0.006590651300513173
1103	7	0.017964638085118168
1103	8	0.02911204075548568
1103	9	-0.005596384186581815
1103	10-11	-6.509414551629789E-4
1103	12-13	-0.017231217778515884
1103	14-15	0.030577911132660063
1103	16-17	0.01308487377946932
1103	18-19	-0.0030017194771474465
1103	20-21	-0.02381038262118551
1103	22-23	-0.10094578847959212
1103	24-25	-0.11238459077777208
1103	26-27	-0.08903040390897132
1103	28-29	-0.07991851264794292
1103	30-31	-0.10626319820448771
1103	32-33	-0.1959896223311759
1103	34-35	-0.1148641684712004
1103	36-37	-0.04398290118856352
1103	38-39	-0.09526132196490522
1103	40-41	-0.08764055639677082
1103	42-43	-0.10741315043538435
1103	44-45	-0.10459824000923845
1103	46-47	-0.10768244814038752
1103	48-49	-0.15736093592947498
1103	50-51	-0.08381995622107041
1103	52-53	-0.06368709824148056
1103	54-55	-0.06292756508163677
1103	56-57	-0.020025894916706477
1103	58-59	-0.09099894864453972
1103	60-61	-0.10999351613349262
1103	62-63	-0.13176231621539358
1103	64-65	-0.2189888966500959
1103	66-67	-0.09827534747028466
1103	68-69	-0.030970570940176856
1103	70-71	-0.06929827946101597
1103	72-73	-0.12296697985190264
1103	74-75	-0.13445202767662323
1103	76-77	-0.10314230162595805
1103	78-79	-0.02056873846229479
1103	80-81	-0.029520375023537326
1103	82-83	0.02202505276343203
1103	84-85	0.03202332913203065
1103	86-87	0.04375006660607994
1103	88-89	-0.0668004222589147
1103	90-91	-0.042531605539956274
1103	92-93	0.021875861339758274
1103	94-95	0.005259567357661865
1103	96-97	0.0131583310030976
1103	98-99	0.03790040343677248
1103	100	0.0538514112811157
1104	1	0.0788283105586558
1104	2	0.027097024183959206
1104	3	-0.012388736190040106
1104	4	0.002368614347410869
1104	5	0.0025388184240782152
1104	6	0.004780058918427699
1104	7	-0.019455342460162228
1104	8	0.015342239812994762
1104	9	-0.04776057291363145
1104	10-11	-0.06097198006662552
1104	12-13	-0.06574517669507429
1104	14-15	0.01696841345069089
1104	16-17	-0.06203461481414507
1104	18-19	-0.06400012447016934
1104	20-21	-0.0699584943898941
1104	22-23	-0.12709640606839656
1104	24-25	-0.10430436695492773
1104	26-27	-0.042567695327399235
1104	28-29	-0.08510350249648013
1104	30-31	-0.0887942697270887
1104	32-33	-0.13986676426915068
1104	34-35	-0.09486195781144602
1104	36-37	-0.06822144263390584
1104	38-39	-0.042666371808586234
1104	40-41	-0.03775849501817419
1104	42-43	-0.05909768305162544
1104	44-45	-0.08464540024335321
1104	46-47	-0.12719714975037988
1104	48-49	-0.026524358684987703
1104	50-51	-0.0720011613988234
1104	52-53	-0.06071280617559438
1104	54-55	-0.1613000029775833
1104	56-57	-0.12715984073819442
1104	58-59	-0.15187833859373967
1104	60-61	-0.15715629866489422
1104	62-63	-0.10810470054962451
1104	64-65	-0.15505482527894543
1104	66-67	-0.1414264786228827
1104	68-69	-0.11320760798059837
1104	70-71	-0.10355106771837796
1104	72-73	-0.12820499206257097
1104	74-75	-0.1711644791910203
1104	76-77	-0.16208521525572195
1104	78-79	-0.11492836487753522
1104	80-81	-0.1667815775570176
1104	82-83	-0.11429888252737896
1104	84-85	-0.10398013276417117
1104	86-87	-0.04859611738517344
1104	88-89	-0.05630994410903867
1104	90-91	-0.09409535823604642
1104	92-93	-0.14055997076177817
1104	94-95	-0.0934077456188831
1104	96-97	-0.06903897730185093
1104	98-99	-0.07122602690230195
1104	100	-0.06357843887860071
1105	1	0.09893456533208678
1105	2	0.08034642595202257
1105	3	0.020768391695824562
1105	4	0.0372054134396862
1105	5	0.01032374840985284
1105	6	0.023300118031023942
1105	7	0.04781874193589175
1105	8	-0.006788124487428604
1105	9	-0.028388372702643494
1105	10-11	-0.00835691790943116
1105	12-13	-0.01113067377353616
1105	14-15	0.061186101704549856
1105	16-17	0.054344634770188804
1105	18-19	-0.05600669433361105
1105	20-21	-0.011917055490435757
1105	22-23	-0.00822942442727026
1105	24-25	-0.0066450107923046176
1105	26-27	-0.046608461757543296
1105	28-29	-0.012181080266636002
1105	30-31	-0.016928098053305973
1105	32-33	0.014714692909457483
1105	34-35	-0.03683987262506605
1105	36-37	-0.04735473136703661
1105	38-39	0.010765012634728066
1105	40-41	0.08009244557228357
1105	42-43	0.05732454910742746
1105	44-45	0.09023960403757769
1105	46-47	0.08805242071272801
1105	48-49	0.14297886905217894
1105	50-51	0.16356836349351767
1105	52-53	0.08922479976432385
1105	54-55	0.14561539645417554
1105	56-57	0.10370482539003234
1105	58-59	0.15282392041787318
1105	60-61	0.2072206215411896
1105	62-63	0.12941822060161456
1105	64-65	0.14905954303318936
1105	66-67	0.17979010155868735
1105	68-69	0.22827230979789448
1105	70-71	0.1406225402874739
1105	72-73	0.09873982501306955
1105	74-75	0.09947108519213543
1105	76-77	0.11319576021762856
1105	78-79	0.16959057114197407
1105	80-81	0.1281308801103478
1105	82-83	0.18606895356573716
1105	84-85	0.17090533369497507
1105	86-87	0.18095134080792263
1105	88-89	0.19567297378384296
1105	90-91	0.2725551321928208
1105	92-93	0.2725290320540452
1105	94-95	0.2464161875871227
1105	96-97	0.12328037167390704
1105	98-99	0.17418234799819032
1105	100	0.2483532696671915
1106	1	0.06431930653207729
1106	2	0.07370877675163712
1106	3	-0.01864552475772996
1106	4	-0.017196947981702237
1106	5	-0.016381556898920735
1106	6	0.013626410587889382
1106	7	0.024069446687015272
1106	8	0.004254099004782574
1106	9	0.05468066693082818
1106	10-11	0.010799612848508389
1106	12-13	-0.016838821177643126
1106	14-15	0.020310247584667707
1106	16-17	-9.83957247697731E-4
1106	18-19	-0.03259192736810945
1106	20-21	-0.05761167552133628
1106	22-23	-0.07237880665156382
1106	24-25	-0.07575988185892868
1106	26-27	-0.1182671717118069
1106	28-29	-0.07663497270573316
1106	30-31	-0.07231172319689705
1106	32-33	-0.11815105127887193
1106	34-35	-0.06426060856804838
1106	36-37	-0.035819326402126705
1106	38-39	-0.042109987124739234
1106	40-41	-0.07439150342009526
1106	42-43	-0.0821992411740311
1106	44-45	-0.09298722287939398
1106	46-47	-0.04827790888951
1106	48-49	-0.0396983000900164
1106	50-51	-0.03970206659228381
1106	52-53	-0.09726793528580657
1106	54-55	-0.08959801084675689
1106	56-57	-0.12880262621064986
1106	58-59	-0.17875508771586368
1106	60-61	-0.1428279653967124
1106	62-63	-0.1929030997315877
1106	64-65	-0.15405944974515506
1106	66-67	-0.09234014222266751
1106	68-69	-0.15857804435758993
1106	70-71	-0.16354073491471155
1106	72-73	-0.1614736303937434
1106	74-75	-0.13308533819347446
1106	76-77	-0.1073066768262052
1106	78-79	-0.12125690050923055
1106	80-81	-0.107080891590698
1106	82-83	-0.055455175381112554
1106	84-85	-0.09557150717269991
1106	86-87	-0.06729423778072885
1106	88-89	-0.06832497113300207
1106	90-91	-0.1563558276734085
1106	92-93	-0.18625683328412634
1106	94-95	-0.1668540115904662
1106	96-97	-0.14594956865555986
1106	98-99	-0.1165722793063182
1106	100	-0.11368272507580102
1107	1	0.03810226541965278
1107	2	0.014027676080665685
1107	3	-0.015662749425558786
1107	4	-0.017555640198303024
1107	5	-0.03457443815127448
1107	6	-0.022313730205567595
1107	7	-0.011937629441497677
1107	8	-0.051812568739258325
1107	9	-0.046610760347746805
1107	10-11	-0.04099709916456362
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2316	14-15	0.09947623095972347
2316	16-17	0.05583219540147155
2316	18-19	0.06546515572379263
2316	20-21	0.08219944445738037
2316	22-23	0.059506495108230695
2316	24-25	0.09530700054567376
2316	26-27	0.09720783439226466
2316	28-29	0.07441164782508025
2316	30-31	-0.24905429900582732
2316	32-33	0.03561579869215592
2316	34-35	-0.045772377582686374
2316	36-37	-0.24532801240503233
2316	38-39	-0.18965961231651818
2316	40-41	-0.11212505249135774
2316	42-43	-0.24834004661995834
2316	44-45	-0.03927540065911472
2316	46-47	-0.14594888667069483
2316	48-49	-0.16005200826801058
2316	50-51	-0.14570829092328808
2316	52-53	-0.15663139904584966
2316	54-55	0.009223994422782766
2316	56-57	-0.6926246132590776
2316	58-59	-0.5846441503126698
2316	60-61	-0.2367887608574719
2316	62-63	0.05304835194420576
2316	64-65	0.1082971956756964
2316	66-67	0.1436494644564661
2316	68-69	-0.03837210937779645
2316	70-71	-0.03290212146826832
2316	72-73	0.022319968166065962
2316	74-75	0.07735900178327171
2316	76-77	0.028251818401685114
2316	78-79	-0.013319785202405399
2316	80-81	-0.035281885005588265
2316	82-83	-0.29276654134480395
2316	84-85	-0.09645814636193961
2316	86-87	-0.16240022997480708
2316	88-89	-0.19442689273120806
2316	90-91	-0.06708715012509714
2316	92-93	-0.11518266243572128
2316	94-95	-0.04042593213184631
2316	96-97	-0.18772911509570633
2316	98-99	0.0172326163413814
2316	100	-0.2395880409022908
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	8.0
13	45.0
14	166.0
15	494.0
16	1234.0
17	2516.0
18	4613.0
19	7177.0
20	10860.0
21	16575.0
22	22021.0
23	23876.0
24	24179.0
25	26458.0
26	31744.0
27	39705.0
28	49587.0
29	61630.0
30	75987.0
31	94577.0
32	120303.0
33	157569.0
34	235337.0
35	354937.0
36	632513.0
37	1289648.0
38	1438639.0
39	234362.0
40	23.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.397393999631106	19.943377924112326	14.080261782447401	23.57896629380917
2	15.81214764064869	20.58481691183665	39.17036615615225	24.432669291362412
3	18.97820748114522	25.48656858664664	30.100988540563574	25.434235391644567
4	13.333634607345813	17.320175728827568	38.55563589529222	30.790553768534405
5	14.141027751684243	37.72753830907442	33.966155340996764	14.165278598244576
6	30.670882301555885	36.77175088857982	18.399503226485873	14.157863583378422
7	27.71778481414869	32.437356068500044	21.757348765379174	18.087510351972096
8	26.900817567874274	34.22806159246687	19.9021742967958	18.96894654286305
9	26.445901168374007	16.21048324749548	20.438797957968234	36.90481762616228
10-11	25.5801572048314	25.74932947921953	27.67448998911895	20.99602332683012
12-13	26.287952026959417	23.139358099929307	27.7324773482161	22.840212524895172
14-15	23.657041685298587	24.70128994245549	25.325698390575397	26.315969981670527
16-17	23.23666612363968	27.51521778492399	25.945358707992533	23.302757383443794
18-19	22.82309860217645	27.065906099803673	27.752576970172843	22.358418327847033
20-21	24.033250238335338	26.124246271745356	27.43315136359285	22.409352126326453
22-23	23.944672964167673	25.87425185821201	27.12759551006994	23.053479667550373
24-25	23.566278632969027	26.020066228398804	27.369014922169328	23.044640216462845
26-27	23.42214732455476	26.32864397988254	27.017440472864042	23.23176822269866
28-29	23.17821387537426	26.514916351669115	27.20907951577557	23.09779025718105
30-31	23.810795796775768	26.034426459867287	27.223148984384228	22.931628758972717
32-33	23.726644158902193	26.267509471975515	26.82195107310383	23.183895296018466
34-35	23.75738050832556	26.4240634508582	27.083528584876337	22.735027455939903
36-37	23.699569794473277	26.226264318283686	26.90258981850619	23.17157606873684
38-39	23.697261859940138	26.207896148170207	27.085303113028736	23.00953887886092
40-41	23.756749953718206	25.769248812436913	27.312291385129594	23.161709848715283
42-43	23.532262473885066	25.91002036737951	27.033161806169936	23.524555352565493
44-45	23.60967829239549	26.187884471621008	26.975794383444786	23.22664285253872
46-47	23.54907996440225	26.042526439561197	26.594237094655437	23.814156501381117
48-49	23.85374792063544	25.92786142203567	26.863851647496347	23.354539009832546
50-51	23.804911401634524	26.098263339082894	26.627163184834313	23.46966207444827
52-53	23.558365905753018	26.24943773549161	26.560805420012972	23.631390938742395
54-55	23.470087304836177	26.07498858578997	26.658117021527715	23.79680708784614
56-57	23.836638672008576	26.061753005328665	26.37542444397864	23.72618387868412
58-59	23.508015527903947	26.214887807625715	26.776838229212103	23.500258435258235
60-61	23.658734713191496	26.330497176031425	26.543962993609444	23.466805117167635
62-63	23.795033181122893	26.087994635177115	26.395162621193712	23.72180956250628
64-65	23.313838441315145	26.007468373800393	26.68060854566841	23.998084639216053
66-67	23.533062784443764	26.005709226486463	26.543777444470013	23.917450544599763
68-69	23.276555661405506	26.039077999847482	26.67457111603643	24.009795222710583
70-71	23.28881482587346	25.829557330019327	26.669131324217126	24.212496519890088
72-73	23.368496549330974	26.0711499052535	26.53787665957686	24.022476885838664
74-75	23.401554315809907	26.188016164174226	26.5654781612354	23.844951358780467
76-77	23.290437169246587	26.12144075508493	26.301373234758675	24.28674884090981
78-79	23.157237041330514	26.06053905611817	26.545599583271212	24.2366243192801
80-81	23.13013711273724	26.40289963576009	26.47980470309187	23.987158548410804
82-83	23.491802537858263	26.036913495016318	26.827588405209994	23.643695561915422
84-85	23.492902087042324	26.132357926997248	26.416669869400096	23.958070116560332
86-87	22.895259434918685	26.44218440971472	26.667865078226615	23.994691077139976
88-89	23.114512481147976	25.888689349587846	26.690967803045396	24.305830366218785
90-91	23.218140827453336	25.90602760867701	26.711077262938097	24.164754300931556
92-93	23.152338063619844	26.01956169622932	26.711739206704333	24.116361033446506
94-95	22.76602954057378	26.029391278085118	26.840268639441117	24.364310541899986
96-97	22.749578700110536	25.938324076807014	26.840661385850172	24.471435837232278
98-99	22.568533109957716	26.07027676928892	26.727834706078585	24.63335541467478
100	22.72281592278687	25.917480889387434	26.576653277301094	24.783049910524607
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	96.0
1	85.0
2	119.0
3	181.0
4	146.0
5	120.5
6	279.5
7	545.5
8	850.0
9	1060.5
10	979.0
11	878.5
12	989.5
13	1169.0
14	1367.0
15	1601.5
16	1885.5
17	2189.0
18	2499.5
19	2825.0
20	3218.5
21	3675.5
22	4228.0
23	4972.0
24	5891.0
25	7177.0
26	8906.5
27	10972.0
28	13723.0
29	17780.0
30	23070.0
31	29805.0
32	39046.0
33	50245.5
34	62142.0
35	74466.0
36	90218.5
37	112124.5
38	129269.0
39	138503.0
40	145514.0
41	151677.5
42	160948.0
43	173628.0
44	186507.5
45	203589.5
46	219387.5
47	230660.5
48	239959.0
49	240900.0
50	235102.0
51	230282.5
52	224189.5
53	215022.0
54	205047.5
55	186192.0
56	164396.0
57	141396.5
58	118970.0
59	102296.0
60	82566.0
61	63735.0
62	50107.0
63	38490.0
64	27673.0
65	18487.5
66	12947.0
67	9589.0
68	7359.0
69	5572.5
70	4140.0
71	3191.0
72	2421.0
73	1797.0
74	1290.5
75	880.5
76	624.5
77	397.5
78	233.0
79	150.0
80	80.5
81	51.0
82	36.0
83	20.0
84	10.5
85	6.0
86	4.5
87	3.0
88	1.5
89	1.0
90	1.5
91	0.5
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3575502351725493
2	4.236616226724333E-4
3	0.0017148208536741348
4	0.001170113053095292
5	0.0052049856499756094
6	0.0
7	2.0174362984401587E-5
8	2.2191799282841743E-4
9	0.0031068518995978445
10-11	9.078463342980713E-5
12-13	4.034872596880317E-5
14-15	2.92528263273823E-4
16-17	7.061027044540555E-5
18-19	8.977591528058705E-4
20-21	1.9165644835181508E-4
22-23	1.0087181492200793E-4
24-25	0.0
26-27	0.001956913209486954
28-29	0.010702499563225042
30-31	0.003278333984965258
32-33	0.014495279804292539
34-35	0.011549822808569907
36-37	0.013446212929103658
38-39	0.013072987213892228
40-41	0.014797895249058564
42-43	0.006677714147836925
44-45	0.0013012464124939024
46-47	0.0059110883544296645
48-49	0.013002376943446824
50-51	0.0035607750667468797
52-53	0.005376467735343023
54-55	0.004942718931178388
56-57	1.412205408908111E-4
58-59	6.455796155008508E-4
60-61	4.1357444118023253E-4
62-63	0.0010490668751888825
64-65	3.5305135222702777E-4
66-67	0.001462641316369115
68-69	0.0016946464906897332
70-71	9.280206972824729E-4
72-73	0.0027941492733396197
74-75	0.007928524652869823
76-77	0.004125657230310125
78-79	0.0017148208536741348
80-81	5.144462561022404E-4
82-83	1.613949038752127E-4
84-85	0.001664384946213131
86-87	0.004539231671490357
88-89	0.0018257798500883435
90-91	2.2191799282841743E-4
92-93	0.0022090927467919735
94-95	0.0036212981557000846
96-97	0.0020779593873933634
98-99	0.0022696158357451784
100	0.002340226106190584
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4956786.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.59668689128912
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.6872914298795	49.53744953036965
2	13.93930080992033	18.00865299803833
3	4.5028284172323945	8.726033921794798
4	1.9054142139364658	4.9233378150346265
5	0.9227621104616924	2.9803687562319547
6	0.5440569924784753	2.1086567516489105
7	0.3447774501954942	1.559003669821876
8	0.25412399668633856	1.3132454596408323
9	0.16028547449295757	0.931851954813896
>10	0.7087717543670664	7.549405235353354
>50	0.022804142219574015	0.9919473373797523
>100	0.007154570700652811	0.8021083674412124
>500	2.1431884933745992E-4	0.09423342397129725
>1k	1.4287905319371766E-4	0.164746815304839
>5k	7.143952659645641E-5	0.30895796315472485
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7337	0.14801930121655443	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6053	0.1221154191445828	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.0174362984401587E-5
2	0.0	0.0	0.0	0.0	2.0174362984401587E-5
3	0.0	0.0	0.0	0.0	2.0174362984401587E-5
4	0.0	0.0	0.0	6.052308895320476E-5	2.0174362984401587E-5
5	0.0	0.0	0.0	1.0087181492200793E-4	2.0174362984401587E-5
6	0.0	0.0	0.0	1.0087181492200793E-4	6.052308895320476E-5
7	0.0	0.0	0.0	1.0087181492200793E-4	8.069745193760635E-5
8	0.0	0.0	0.0	1.0087181492200793E-4	1.0087181492200793E-4
9	0.0	0.0	0.0	2.824410817816222E-4	1.0087181492200793E-4
10-11	0.0	0.0	0.0	3.631385337192285E-4	1.0087181492200793E-4
12-13	0.0	0.0	0.0	3.833128967036301E-4	1.5130772238301188E-4
14-15	0.0	0.0	0.0	4.236616226724333E-4	2.2191799282841743E-4
16-17	0.0	0.0	0.0	4.640103486412365E-4	2.824410817816222E-4
18-19	0.0	0.0	0.0	5.245334375944412E-4	3.026154447660238E-4
20-21	0.0	0.0	0.0	7.363642489306579E-4	3.4296417073482696E-4
22-23	2.0174362984401587E-5	0.0	0.0	8.77584789821469E-4	3.7322571521142934E-4
24-25	2.0174362984401587E-5	0.0	0.0	0.001119677145634288	3.8331289670363015E-4
26-27	2.0174362984401587E-5	0.0	0.0	0.001553425949798922	3.8331289670363015E-4
28-29	2.0174362984401587E-5	0.0	0.0	0.0026024928249878047	4.1357444118023253E-4
30-31	2.0174362984401587E-5	0.0	0.0	0.005971611443382869	4.337488041646341E-4
32-33	2.0174362984401587E-5	0.0	0.0	0.015191295327254394	4.640103486412365E-4
34-35	2.0174362984401587E-5	0.0	0.0	0.030977734362548637	4.8418471162563805E-4
36-37	2.0174362984401587E-5	0.0	0.0	0.05654873944527765	4.8418471162563805E-4
38-39	2.0174362984401587E-5	0.0	0.0	0.09683694232512761	4.8418471162563805E-4
40-41	2.0174362984401587E-5	0.0	0.0	0.1448721005909878	4.942718931178388E-4
42-43	2.0174362984401587E-5	0.0	0.0	0.1971337879020801	5.144462561022404E-4
44-45	2.0174362984401587E-5	0.0	0.0	0.2619439289894702	5.447078005788428E-4
46-47	2.0174362984401587E-5	0.0	0.0	0.3408761241659414	5.648821635632444E-4
48-49	2.0174362984401587E-5	0.0	0.0	0.4285034697886897	5.951437080398468E-4
50-51	2.0174362984401587E-5	0.0	0.0	0.5220318165843754	6.052308895320476E-4
52-53	2.0174362984401587E-5	0.0	0.0	0.6168210610665863	6.254052525164493E-4
54-55	2.0174362984401587E-5	0.0	0.0	0.7299992374090791	6.455796155008508E-4
56-57	2.0174362984401587E-5	0.0	0.0	0.857642432011388	6.455796155008508E-4
58-59	2.0174362984401587E-5	0.0	0.0	1.0060248717616616	6.455796155008508E-4
60-61	2.0174362984401587E-5	0.0	0.0	1.163334467132533	6.859283414696539E-4
62-63	2.0174362984401587E-5	0.0	0.0	1.3304891516397923	6.859283414696539E-4
64-65	2.0174362984401587E-5	0.0	0.0	1.5013155702102128	6.859283414696539E-4
66-67	2.0174362984401587E-5	0.0	0.0	1.6938697777148337	6.859283414696539E-4
68-69	2.0174362984401587E-5	0.0	0.0	1.9152027140167034	6.859283414696539E-4
70-71	2.0174362984401587E-5	0.0	0.0	2.154551356463644	7.061027044540555E-4
72-73	2.0174362984401587E-5	0.0	0.0	2.40222192364165	7.76712974899461E-4
74-75	2.0174362984401587E-5	0.0	0.0	2.6532212607120824	8.170617008682643E-4
76-77	2.0174362984401587E-5	0.0	0.0	2.931264331363105	8.674976083292682E-4
78-79	2.0174362984401587E-5	0.0	0.0	3.2391755464125342	9.078463342980713E-4
80-81	2.0174362984401587E-5	0.0	0.0	3.574039710409124	9.28020697282473E-4
82-83	2.0174362984401587E-5	0.0	0.0	3.91778059411885	9.481950602668746E-4
84-85	2.0174362984401587E-5	0.0	0.0	4.270216628274854	9.683694232512761E-4
86-87	2.0174362984401587E-5	0.0	0.0	4.633869608250185	9.986309677278784E-4
88	2.0174362984401587E-5	0.0	0.0	4.927769728206947	0.0010288925122044809
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7020	0.0	47.50502	1
GTATCAA	11870	0.0	40.016212	1
CAACGCA	14060	0.0	32.624325	5
ATCAACG	14335	0.0	31.932577	3
TATCAAC	15160	0.0	31.278904	2
AACGCAG	15770	0.0	29.59189	6
TCAACGC	15775	0.0	29.047737	4
GTACTAG	2855	0.0	26.599716	1
ACGCAGA	17770	0.0	25.732418	7
CGCAGAG	18380	0.0	24.776379	8
GCAGAGT	20590	0.0	21.843143	9
GCATCGT	3040	0.0	21.79742	8
GTACATG	20085	0.0	21.79382	1
TACATGG	20175	0.0	21.12775	2
GTGGTAT	2960	0.0	20.875496	1
CGTTAAC	770	0.0	20.215311	1
ACATGGG	20555	0.0	19.777496	3
CTAGTAC	3675	0.0	19.182562	3
GAGTACT	8550	0.0	19.127365	12-13
TAGTACT	3635	0.0	18.876677	4
>>END_MODULE