FastQCFastQC Report
Thu 26 May 2016
ERR580230_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580230_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4245284
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145290.3422385875715264No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123560.29105237717900617No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71710.16891684985032804No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63650.054.9034961
GTATCAA118650.042.8120081
CAACGCA143100.034.6587075
ATCAACG143850.034.5082443
TATCAAC148850.033.862762
TCAACGC152200.032.77184
AACGCAG154400.032.468386
ACGCAGA172500.028.7902037
CGCAGAG175600.028.043128
GTACATG141800.026.6015971
TACATGG143250.025.7990842
GTGGTAT26150.025.3604551
ACATGGG144150.025.0120643
GCAGAGT195600.024.4347699
CATGGGG112150.023.4321444
ATGGGGG84750.022.9646665
CTAACGC7800.021.695853
TGGTATC28250.021.637212
GAGTACT125000.021.26113312-13
CAGAGTA191300.021.18912710-11
Produced by FastQC (version 0.11.5)