FastQCFastQC Report
Thu 26 May 2016
ERR580230_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580230_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4245284
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129800.3057510404486484No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117540.27687193601181925No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69730.16425285092823No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68350.053.8642351
GTATCAA118450.039.3383261
ATCAACG145150.031.3463613
CAACGCA145350.031.3337235
TATCAAC151000.030.7976152
TCAACGC153600.029.8044574
AACGCAG156350.029.6388826
GTGGTAT28400.028.4765471
GTACATG136300.027.1836381
TACATGG138650.026.4867612
ACGCAGA174800.026.0803327
CGCAGAG177500.025.6032758
ACATGGG139950.025.290123
TGGTATC30650.025.1600272
CATGGGG107150.024.827774
ATGGGGG78550.023.5750855
GCAGAGT197850.022.5884449
TGGGGGG76100.021.9243376
GAGTACT126200.020.77825212-13
CAGAGTA194750.020.42613810-11
Produced by FastQC (version 0.11.5)