FastQCFastQC Report
Thu 26 May 2016
ERR580229_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580229_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3243772
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53500.16493144401024487No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42940.13237675151027878No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53800.045.6199721
GTATCAA95450.039.0135961
CAACGCA111500.032.298865
ATCAACG112550.031.8732013
TATCAAC120950.030.5166762
AACGCAG124300.029.60356
TCAACGC124300.029.3141464
GTACTAG15400.028.0888441
GTACATG183050.027.2015061
ACGCAGA137900.026.0015537
TACATGG191050.025.7429372
ACATGGG188350.025.2616423
CGCAGAG142050.025.0761178
CATGGGG132900.023.4903644
GCAGAGT154400.022.4944979
ATGGGGG92700.021.5547245
TGGGGGG80900.020.9706126
CATGGGT19650.020.5770234
GCATCGT18100.019.9914598
TAGTACT22050.019.8299224
Produced by FastQC (version 0.11.5)