FastQCFastQC Report
Thu 26 May 2016
ERR580229_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580229_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3243772
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49650.15306254570296554No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40080.12355985562487129No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50400.047.5388031
GTATCAA94250.041.134431
CAACGCA110650.033.8521355
ATCAACG113400.033.032733
TATCAAC116900.032.4854852
AACGCAG122750.031.4311756
TCAACGC121600.030.9974964
GCGTAAT2002.5465852E-1128.2986871
TACCTGG17950.028.2782042
GTACATG181100.027.6059341
ACGCAGA138700.026.9696667
GTACTAG16600.026.4234731
TACATGG189000.026.110792
ACATGGG185900.026.0409623
CGCAGAG142750.025.974068
CTGTGCG22400.024.125829
GCAGAGT155000.023.6177239
TAGGCAT24550.023.5468465
CATGGGG130700.023.4813694
TAGTACT22950.022.9361824
Produced by FastQC (version 0.11.5)