FastQCFastQC Report
Thu 26 May 2016
ERR580228_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580228_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11329836
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT261060.230418163157878No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200110.17662215057658381No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122650.10825399414431064No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA177400.043.617581
GTATCAA310050.039.1111451
CAACGCA333150.034.429385
GTACTAG104100.033.7083471
ATCAACG346400.033.1739233
GGGCACC115450.031.7124894
TATCAAC389100.030.8758262
GCATCGT106100.030.6113178
AACGCAG392700.030.2469046
TAGGCAT133800.028.8213925
CTGTGCG143450.028.4079429
ACGCAGA405950.028.2407257
GTGGTAT51600.027.9858191
TCAACGC420250.027.6336174
CGCAGAG414400.027.607068
TCTAACG50200.027.548792
TAGTACT149850.027.4333654
GTACATG482800.027.2894741
CTAACGC51300.026.7742123
TACATGG488950.026.5139052
Produced by FastQC (version 0.11.5)