FastQCFastQC Report
Thu 26 May 2016
ERR580228_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580228_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11329836
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT232330.20506033803137136No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183780.1622088792812182No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117660.10384969385258533No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA177200.045.4451831
GTATCAA310250.038.6841661
CAACGCA341750.033.5160645
GTACTAG105250.033.2757871
ATCAACG349650.032.7722433
GGGCACC118200.031.89596494
TATCAAC391850.030.4308662
AACGCAG402850.029.317856
TAGGCAT131200.029.1247835
TCAACGC423500.027.2903544
CTGTGCG149750.027.2418069
ACGCAGA423050.027.0624227
TAGTACT154200.026.9141374
GTACATG476600.026.6320081
CTAGGCA149250.026.452644
CGCAGAG433250.026.4252958
GTGGTAT51150.026.3773081
TACATGG478850.026.0504422
ACATGGG465700.025.8671383
GCATCGT130350.025.4911548
Produced by FastQC (version 0.11.5)