FastQCFastQC Report
Thu 26 May 2016
ERR580227_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580227_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6313388
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89140.1411920192454511No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70060.11097052802710684No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87200.045.9425471
GTATCAA150000.040.6261561
CAACGCA177700.033.097865
ATCAACG177900.032.9316443
TATCAAC191250.031.6915472
TCAACGC198000.030.0385824
AACGCAG199900.029.8564766
ACGCAGA225200.026.0010197
CGCAGAG231950.025.183388
GTACTAG31500.025.0778731
GTACATG275300.023.7069241
TACATGG277150.023.2093622
ACATGGG283250.022.044463
GCAGAGT257150.022.006259
TAGTACT42850.021.9448834
GTATAAG35350.021.4155121
TGATCGC21750.020.5291259
GCATCGT32900.020.2828858
CTAGTAC47700.020.207233
GTATATA23450.020.0516051
Produced by FastQC (version 0.11.5)