FastQCFastQC Report
Thu 26 May 2016
ERR580227_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580227_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6313388
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78750.1247349283776001No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67330.10664638384335003No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87700.045.056821
GTATCAA152950.040.6949581
CAACGCA178950.033.6418275
ATCAACG180850.033.315433
TATCAAC191550.032.31242
AACGCAG198850.030.8886136
TCAACGC199550.030.4289284
ACGCAGA224050.026.764197
CGCAGAG230700.025.8095728
GTACTAG33050.025.3959621
GTACATG272900.022.998031
GCAGAGT258450.022.5474269
TACATGG274550.022.5439452
GTGGTAT41350.021.4387021
ACATGGG281250.021.1217773
CTGTGCG45600.019.9934969
TAGTACT46350.019.9752584
CTAGTAC48150.019.814163
TAGGCAT47650.019.4296725
GAGTACT98050.019.41104512-13
Produced by FastQC (version 0.11.5)