FastQCFastQC Report
Thu 26 May 2016
ERR580226_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580226_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7580594
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270620.3569904944124431No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216140.28512277533924124No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123080.16236194683424543No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA147650.052.5446931
GTATCAA270800.041.37651
CAACGCA314150.034.2173845
GTGGTAT45050.033.9208641
ATCAACG321700.033.397963
TATCAAC342200.032.349162
GTACTAG54800.031.832571
AACGCAG347450.031.520276
TCAACGC357850.030.2750434
ACGCAGA372900.028.6518297
CGCAGAG377050.028.249068
TAGGCAT71000.026.9434855
GTACATG358000.026.1890961
GCATCGT59600.026.0935488
CTAGGCA75200.025.7510074
GCAGAGT408100.025.5613429
TACATGG364200.025.3614862
ACATGGG360700.024.978753
TGGTATC62500.024.2174262
GCTATTG69550.023.5089668
Produced by FastQC (version 0.11.5)