FastQCFastQC Report
Thu 26 May 2016
ERR580226_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580226_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7580594
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230590.30418460611397996No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197970.26115367740311646No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115010.15171634307285156No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA141700.051.991131
GTATCAA261100.041.990571
CAACGCA311550.034.1704145
ATCAACG316300.033.687873
GTGGTAT46750.032.8848761
TATCAAC337150.032.352392
AACGCAG347750.031.3403076
GTACTAG56050.031.0353161
TCAACGC354050.030.3343184
ACGCAGA371650.028.5787967
CGCAGAG378700.027.934898
TAGGCAT71950.026.3259455
GTACATG358700.025.715661
GCAGAGT408850.025.5744929
GGGCACC66400.025.48060894
CTGTGCG75800.025.1708459
TAGTACT76700.025.124714
TACATGG366450.024.851722
CTAGGCA75800.024.8029544
ACATGGG361850.024.5111983
Produced by FastQC (version 0.11.5)