FastQCFastQC Report
Thu 26 May 2016
ERR580225_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580225_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5832907
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60050.10295038134501373No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85400.043.219751
GTATCAA151750.040.713381
CAACGCA167700.034.8465775
ATCAACG177650.032.9255563
TATCAAC188200.032.504752
AACGCAG211650.029.5086926
GTGGTAT25900.028.1385941
GTACTAG44900.026.9126991
ACGCAGA214000.026.900367
CGCAGAG217350.026.3339468
TACCCCG4850.026.172435
TCAACGC230950.025.7335434
AAGCTAA42650.025.3459119
TAGTACT55500.024.6530384
CTGTGCG55700.023.964169
GTACATG295650.023.6007861
GTGATCG41300.023.553138
GCAGAGT239200.023.5197149
TACATGG298100.023.0765822
GCATCGT47400.022.5048358
Produced by FastQC (version 0.11.5)